H2AX Polyclonal Antibody

供应商 ：Bio-Swamp

库存 ：800

靶点 ：H2AX

克隆性 ：多克隆

抗体英文名 ：H2AX Antibody

抗体名 ：H2AX 抗体

宿主 ：Rabbit

适应物种 ：Human,Mouse,Rat

亚型 ：IgG

应用范围 ：WB,IHC,ELISA

Product Information： 

 

Product name	H2AX Polyclonal Antibody
Isotype	IgG
Form	Lyophilized or Liquid
Tested applications	WB,IHC,ELISA
Species reactivity	Human,Mouse,Rat
Host Species	Rabbit
Purity	Affinity purification

 

Immunogen Information：

 

Immunogen	Recombinant protein of human H2AX
Calculated molecular weight	15kDa
Observed molecular weight	16kDa
Swiss Prot	P16104
Gene ID (NCBI)	3014
Alternative Names	H2A.X;H2A/X;H2AX

 

Application Notes：

 

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB	1:500 - 1:2000
IHC	1:50 - 1:200

 

                                          

Western blot analysis of extracts of various cell lines, using H2AX antibody.

Immunohistochemistry of paraffin-embedded rat testis using H2AX antibody.

 

                               

Immunohistochemistry of paraffin-embedded mouse testis using H2AX antibody.

Immunohistochemistry of paraffin-embedded mouse brain using H2AX antibody.

 

Background：

 

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.  

 

Storage：

 

Storage instructions

Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.