样本 ：血清，血浆，细胞培养上清及其他生物学样本

库存 ：99

标记物 ：HRP

适应物种 ：Mouse

应用 ：ELISA

检测方法 ：双抗夹心法

检测限 ：23.44 pg/mL - 1500 pg/mL

供应商 ：联科生物

规格 ： 96T

【背景介绍】白细胞介素1(IL-1)是17kDa的胞外多肽，分为IL-1α和IL-1β两种蛋白。IL-1β是活化的巨噬细胞分泌的蛋白前体，通过caspase1(CASP1/ICE)水解产生的活化形式。该细胞因子是炎症反应的重要介导者，参与许多细胞活性，包括细胞增殖、分化和凋亡。IL-1是一类重要的蛋白家族，它们由生物活化的单核细胞所衍生，参与炎症反应和免疫应答。

 

IL-1β表达升高会产生各种不同的自身炎症综合征，最显著的是单基因条件称为CAPS，是由于触发IL-1β加工的炎性体受体NLRP3发生突变而引起的。

 

 

【DESCREPTION】Interleukin-1 (IL-1) is an extracellular peptide of 17 kDa that designates two proteins, IL-1α and IL-1β. IL-1β is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The interleukin-1 (IL-1) species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses.

 

Increased production of IL-1β causes a number of different autoinflammatory syndromes, most notably the monogenic conditions referred to as CAPS, due to mutations in the inflammasome receptor NLRP3 which triggers processing of IL-1β.

 

 

【检测原理】本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-1β单克隆抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-1β与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-1β的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570-630nm)测定吸光度值。

 

【PRINCIPLE OF THE ASSAY】This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse IL-1β has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for IL-1β are pipetted into the wells and IL-1β present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-1β bound in the initial step. The color development is stopped and the intensity of the color is measured.

 

【分析类型】夹心ELISA

【样本类型】血清、血浆、细胞上清

【板式】96孔板，可拆

【保存】试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。

【运输条件】4℃

【样本体积】10 μl

【灵敏度】1.45pg/ml

【标准曲线范围】23.44-1500pg/ml

【回收率范围】83%-109%

【平均回收率】0.97

【板间变异系数】2.8% -5.6 %

【板内变异系数】4.2 % - 8.4%

IL1B 分子靶点信息概述

• 分子名：IL1B, interleukin 1 beta
• 基因家族：Interleukins
• 别名：IL1F2; IL-1B; IL1-BETA

IL1B 分子靶点综述

白细胞介素1(IL-1)是17kDa的胞外多肽，分为IL-1α和IL-1β两种蛋白。IL-1β是活化的巨噬细胞分泌的蛋白前体，通过caspase1(CASP1/ICE)水解产生的活化形式。该细胞因子是炎症反应的重要介导者，参与许多细胞活性，包括细胞增殖、分化和凋亡。IL-1是一类重要的蛋白家族，它们由生物活化的单核细胞所衍生，参与炎症反应和免疫应答。IL-1β表达升高会产生各种不同的自身炎症综合征，最显著的是单基因条件称为CAPS，是由于触发IL-1β加工的炎性体受体NLRP3发生突变而引起的。本试剂盒用来检测人IL-1β的成熟形式和未裂解的前体形式。

小鼠 Mouse Il1b 分子靶点信息

• 分子名：Il1b, interleukin 1 beta
• 别称：
  • IL-1B
  • Il-1b
  • IL-1beta
• 基因序列：NCBI_Gene: 16176
• 蛋白序列：
  • UniprotKB: P10749
  • UniprotKB: Q3TI17

小鼠 Mouse Il1b 靶点分子功能（预测）

Enables cytokine activity. Involved in several processes, including negative regulation of cell communication; regulation of defense response; and regulation of lipid metabolic process. Acts upstream of or within several processes, including ectopic germ cell programmed cell death; positive regulation of intracellular signal transduction; and positive regulation of macromolecule metabolic process. Located in extracellular space and secretory granule. Is expressed in several structures, including dorsal aorta; early conceptus; hemolymphoid system; integument; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); blood platelet disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and lung disease (multiple). Orthologous to human IL1B (interleukin 1 beta).