MMP2 Polyclonal Antibody

供应商 ：Bio-Swamp

库存 ：800

靶点 ：MMP2

克隆性 ：多克隆

抗体英文名 ：MMP2 Antibody

抗体名 ：MMP2 抗体

宿主 ：Rabbit

适应物种 ：Human,Mouse,Rat

亚型 ：IgG

应用范围 ：WB,IHC,IF,ELISA

规格 ：10 µl / 20 µl / 50 µl / 100 µl

Product Information： 

Product name	MMP2 Polyclonal Antibody
Isotype	IgG
Form	Lyophilized or Liquid
Tested applications	WB,IHC,IF,ELISA
Species reactivity	Human,Mouse,Rat
Host Species	Rabbit
Purity	Affinity purification

 

Immunogen Information：

 

Immunogen	Recombinant protein of human MMP2
Calculated molecular weight	65kDa/68kDa/73kDa
Observed molecular weight	72kDa
Swiss Prot	P08253
Gene ID (NCBI)	4313
Alternative Names	CLG4;CLG4A;MMP-2;MMP-II;MONA;TBE-1

 

Application Notes：

 

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB	1:500 - 1:2000
IHC	1:50 - 1:200
IF	1:50 - 1:200

                          

Western blot analysis of extracts of various cell lines, using MMP2 antibody.

Immunohistochemistry of paraffin-embedded human lung cancer using MMP2 Antibody.

                  

Immunohistochemistry of paraffin-embedded human breast cancer using MMP2 Antibody.

Immunohistochemistry of paraffin-embedded human placenta using MMP2 Antibody.

                                                    

 

Immunohistochemistry of paraffin-embedded human mammary cancer using MMP2 antibody.

Immunofluorescence analysis of U2OS cells using MMP2 antibody.

 

Background：

 

This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. 

 

Storage：

 

Storage instructions

Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.