产品详情
文献和实验
相关推荐
保存条件 ：负20摄氏度
保质期 ：6month
英文名 ：pME6032
库存 ：货源充足
供应商 ：LSM Bio
CAS号 ：无
规格 ：2ug
pME6032
Search name
pME6032,Plasmid pME6032,pME6032 vector
pME6032 Information
Promoter: Tac
Replicator: pVS1 ori, p15A ori
Terminator: T4
Plasmids: broad host expression plasmids
Plasmid size: 9816bp
Prokaryotic resistance: tetracycline Tetracyclin
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Host: a wide host
Culture conditions: Reference Literature
5'sequencing primers: Based on full sequence design
3'sequencing primers: Reference Literature
Note: GenBank: DQ645594.1
pME6032 Description
PME6032 can be found in many hosts by using pVS1 and p15A replicas, and can express the protein well with the strong promoter of Tac.
pME6032 Sequence
LOCUS Exported 9816 bp ds-DNA circular SYN 10-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pME6032
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9816)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Saturday, September 10, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..9816
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1180..1809
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/note="pVS1 StaA"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 2238..3311
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/note="pVS1 RepA"
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 3377..3571
/note="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 4281..4826
/direction=RIGHT
/note="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
misc_feature 5148..5414
/note="T4 terminator"
misc_feature 5416..5473
/note="MCS"
misc_feature 5478..5481
/note="RBS"
protein_bind 5490..5506
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
misc_feature complement(5514..5542)
/note="Tac promoter"
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
promoter 5776..5853
/gene="lacI (mutant)"
/note="lacIq promoter"
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
misc_feature 5977..6792
/note="LacIQ"
CDS complement(7821..8471)
/codon_start=1
/gene="tetR"
/product="tetracycline resistance regulatory protein"
/note="TetR"
/translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
CDS 8577..9776
/codon_start=1
/gene="tetA"
/product="tetracycline efflux protein"
/note="TcR"
/note="confers resistance to tetracycline"
/translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
"
ORIGIN
1 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
61 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
121 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
181 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
241 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
301 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
361 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
421 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
481 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
武汉维克赛思科技有限公司
实名认证
钻石会员
入驻年限：6年
