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保存条件 ：负20摄氏度
保质期 ：6month
英文名 ：pNZ8048
库存 ：货源充足
供应商 ：LSM Bio
CAS号 ：无
规格 ：2ug
pNZ8048 (下架） 推荐：pNZ8148 Plasmid
Search name
pNZ8048,Plasmid pNZ8048,pNZ8048 vector
pNZ8048 Plasmid information
Promoter: PnisA
Replicon: repA, repC
Terminator: Terminator
Plasmid classification: secreted expression plasmid of Lactobacillus
Plasmid size: 3349bp
Prokaryotic resistance: chloramphenicol chloramphenicol
Screening marker: chloramphenicol chloramphenicol
Clone strain: DH5 alpha
Culture conditions: 37, aerobic, LB
Expressing host: Lactobacillus
Primers for 5'sequencing: Based on sequence design
pNZ8048 plasmid Sequence
LOCUS Exported 3349 bp ds-DNA circular SYN 10-MAY-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pNZ8048
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3349)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, May 10, 2016 from SnapGene Viewer 3.1.2
FEATURES Location/Qualifiers
source 1..3349
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 6..189
/note="PnisA"
misc_feature 202..252
/note="MCS"
misc_feature 394..446
/note="Terminator"
misc_feature 788..997
/note="RepC"
misc_feature 1266..1964
/note="RepA"
gap 2138..3349
/estimated_length="1212"
ORIGIN
1 agatctagtc ttataactat actgacaata gaaacattaa caaatctaaa acagtcttaa
61 ttctatcttg agaaagtatt ggtaataata ttattgtcga taacgcgagc ataataaacg
121 gctctgatta aattctgaag tttgttagat acaatgattt cgttcgaagg aactacaaaa
181 taaattataa ggaggcactc accatgggta ctgcaggcat gcggtaccac tagttctaga
241 gagctcaagc tttctttgaa ccaaaattag aaaaccaagg cttgaaacgt tcaattgaaa
301 tggcaattaa acaaattaca gcacgtgttg ctttgattga tagccaaaaa gcagcagttg
361 ataaagcaat tactgatatt gctgaaaaat tgtaatttat aaataaaaat caccttttag
421 aggtggtttt tttatttata aattattcgt ttgatttcgc tttcgataga acaatcaaat
481 cgtttctgag acgttttagc gtttatttcg tttagttatc ggcataatcg ttaaaacagg
541 cgttatcgta gcgtaaaagc ccttgagcgt agcgtggctt tgcagcgaag atgttgtctg
601 ttagattatg aaagccgatg actgaatgaa ataataagcg cagcgtcctt ctatttcggt
661 tggaggaggc tcaagggagt ttgagggaat gaaattccct catgggtttg attttaaaaa
721 ttgcttgcaa ttttgccgag cggtagcgct ggaaaatttt tgaaaaaaat ttggaatttg
781 gaaaaaaatg gggggaaagg aagcgaattt tgcttccgta ctacgacccc ccattaagtg
841 ccgagtgcca atttttgtgc caaaaacgct ctatcccaac tggctcaagg gtttgagggg
901 tttttcaatc gccaacgaat cgccaacgtt ttcgccaacg ttttttataa atctatattt
961 aagtagcttt atttttgttt ttatgattac aaagtgatac actaatttta taaaattatt
1021 tgattggagt tttttaaatg gtgatttcag aatcgaaaaa aagagttatg atttctctga
1081 caaaagagca agataaaaaa ttaacagata tggcgaaaca aaaagatttt tcaaaatctg
1141 cggttgcggc gttagctata gaagaatatg caagaaagga atcagaacaa aaaaaataag
1201 cgaaagctcg cgtttttaga aggatacgag ttttcgctac ttgtttttga taaggtaatt
1261 atatcatggc tattaaaaat actaaagcta gaaattttgg atttttatta tatcctgact
1321 caattcctaa tgattggaaa gaaaaattag agagtttggg cgtatctatg gctgtcagtc
1381 ctttacacga tatggacgaa aaaaaagata aagatacatg gaatagtagt gatgttatac
1441 gaaatggaaa gcactataaa aaaccacact atcacgttat atatattgca cgaaatcctg
1501 taacaataga aagcgttagg aacaagatta agcgaaaatt ggggaatagt tcagttgctc
1561 atgttgagat acttgattat atcaaaggtt catatgaata tttgactcat gaatcaaagg
1621 acgctattgc taagaataaa catatatacg acaaaaaaga tattttgaac attaatgatt
1681 ttgatattga ccgctatata acacttgatg aaagccaaaa aagagaattg aagaatttac
1741 ttttagatat agtggatgac tataatttgg taaatacaaa agatttaatg gcttttattc
1801 gccttagggg agcggagttt ggaattttaa atacgaatga tgtaaaagat attgtttcaa
1861 caaactctag cgcctttaga ttatggtttg agggcaatta tcagtgtgga tatagagcaa
1921 gttatgcaaa ggttcttgat gctgaaacgg gggaaataaa atgacaaaca aagaaaaaga
1981 gttatttgct gaaaatgagg aattaaaaaa agaaattaag gacttaaaag agcgtattga
2041 aagatacaga gaaatggaag ttgaattaag tacaacaata gatttattga gaggagggat
2101 tattgaataa ataaaagccc ccctgacgaa agtcgacnnn nnnnnnnnnn nnnnnnnnnn
2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnn
//
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