库存 ：货源充足

供应商 ：LSM Bio

检测限 ：参考说明书

检测方法 ：夹心法/竞争法

应用 ：夹心法/竞争法ELISA体外定量检测小鼠血清、血浆或其它相关生物液体中蛋白浓度

适应物种 ：人

样本 ：血清、血浆或其它相关生物液体中天然和重组蛋白

规格 ：96T

IGFBP-3 ELISA KIT|Human

Catalog  EF000138

Packing 48T/96T

Reactivity Human

Detect Range 0.781-50ng/ml

IGFBP-3 ELISA KIT Sensitivity< 0.469ng/ml

Application IGFBP-3 ELISA KIT|Human  is for detecting the concentration of Human IGFBP-3 in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

 

Principle of IGFBP-3 ELISA KIT|Human

The microtiter plate provided in IGFBP-3 ELISA KIT|Human has been pre-coated with an Human IGFBP-3 antibody specific to Human IGFBP-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human IGFBP-3antibody preparation specific for Human IGFBP-3 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human IGFBP-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human IGFBP-3in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

*Please noted that IGFBP-3 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!