300-02

Immune Interferon,type II interferon,T cell interferon,MAF

IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-γ species-specific and is biologically active only in human and primate cells. Recombinant human IFN-γ is a 16.7 kDa protein containing 143 amino acid residues.

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

E.coli

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED₅₀ for this effect is 5.0-10.0 ng/ml.
Assay #2: The ED₅₀ was determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.


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耗材：细胞培养耗材、普通实验耗材。
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抗体：免疫组化、免疫荧光、流式细胞检测、免疫细胞化学。


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