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英文名 :ExcelBand™ 3-color Pre-stained Protein Ladder, High Range (9-245 kDa), 250 μl x 2
规格 :250 μl x 2
蛋白marker(3色红蓝绿),10-245KDaExcelBand™ 3-color Pre-stained Protein Ladder, High Range (9-245 kDa), 250 μl x 2
Description
The PM5100 3-color Pre-Stained Protein Ladder High Range is a ready-to-use three-color protein standard with 14 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5100 3-color Pre-Stained Protein Ladder High Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
- Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
- Three reference bands — 75 kDa (red), 40 kDa (green), and 20 kDa (blue)
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Quality Control
Under suggested conditions, PM5100 ExcelBand™ 3-color Pre-Stained Protein Ladder High Range resolves 14 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane.
Specification
Cat. No. | PM5100 |
Series Name | ExcelBand™ |
Product Size | 2 x 250 μl |
MW Range | 10 – 245 kDa |
Band Number | 14 |
Band Color | Red/Green/Blue |
Markered Bands | 75, 40, 20 kDa Migration patterns and approximate MWs (kDa)
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ExcelBand 三彩预染型蛋白质常规分子量标记
◆ 高范围即用型产品
◆ 内含14条预染蛋白条带
◆ 其中蓝色蛋白加量条带为20KDa、绿色条带为40KDa、
红色条带为75KDa
◆ 片段范围:10-245KDa
上海研卉生物科技有限公司
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入驻年限:10年