RPMI 2650

运输方式 ：冻存运输

库存 ：大量

细胞形态 ：上皮样

年限 ：52 years

相关疾病 ：鳞状细胞癌

器官来源 ：其他

ATCC Number ：CCL-30™

生长状态 ：贴壁生长

物种来源 ：人

是否是肿瘤细胞 ：1

Designations:	RPMI 2650
Depositors:	GE Moore
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens
Morphology:	epithelial
Source:	Organ: nasal septum Disease: squamous cell carcinoma Derived from metastatic site: pleural effusion
Cellular Products:	mucoid; keratin
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:	The cells are positive for keratin by immunoperoxidase staining.
DNA Profile (STR):	Amelogenin: X,Y CSF1PO: 9,11 D13S317: 11,12 D16S539: 11,12 D5S818: 12,13 D7S820: 8,11 THO1: 6,8 TPOX: 8 vWA: 16,18
Isoenzymes:	G6PD, B
Age:	52 years
Gender:	male
Comments:	The cells are positive for keratin by immunoperoxidase staining.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:	Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal. 4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37�C.NOTE: Cells attach in clusters. Cells will pile and the culture does not get 100% confluent. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020
References:	22218: Moore GE, Sandberg AA. Studies of a human tumor cell line with a diploid karyotype. Cancer 17: 170-175, 1964. PubMed: 14123677 25971: Moorhead PS. Human tumor cell line with a quasi-diploid karyotype (RPMI 2650). Exp. Cell Res. 39: 190-196, 1965. PubMed: 5831238