SV40 MES 13

生长状态 ：贴壁生长

ATCC Number ：CRL-1927™

物种来源 ：小鼠

是否是肿瘤细胞 ：0

组织来源 ：glomerulus

细胞类型 ：其他细胞类型

库存 ：大量

年限 ：7 to 10 weeks

细胞形态 ：其他

器官来源 ：肾脏

运输方式 ：冻存运输

Designations:	SV40 MES 13
Depositors:	LJ Striker
Biosafety Level:	2 [Cells contain polyomavirus DNA sequences ]
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Mus musculus
Morphology:	myofibroblast-like
Source:	Organ: kidney Tissue: glomerulus Cell Type: mesangial cell;
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age:	7 to 10 weeks
Comments:	The cells display prominent cytoskeletal staining for actin, and have abundant parallel fibrils in the cytoplasm. They are reported to contract in the presence of 1 X 10(-6) M angiotensin II. The cells form colonies in soft agar, and are positive for SV40 large T antigen.
Propagation:	ATCC complete growth medium: The base medium for this cell line is 3:1 mixture of ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002 and Ham's F12 medium with 14 mM HEPES.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%. Temperature: 37.0°C
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37�C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Every 2 to 3 days
Preservation:	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Doubling Time:	26 hrs
Related Products:	recommended serum:ATCC 30-2020
References:	22754: MacKay K, et al. Glomerular epithelial, mesangial, and endothelial cell lines from transgenic mice. Kidney Int. 33: 677-684, 1988. PubMed: 2835539 51029: Robey RB, et al. Regulation of mesangial cell hexokinase activity by PKC and the classic MAPK pathway. Am. J. Physiol. 277: F742-F749, 1999. PubMed: 10564237 51030: Robey RB, et al. Thrombin is a novel regulator of hexokinase activity in mesangial cells. Kidney Int. 57: 2308-2318, 2000. PubMed: 10844601 61211: Robey RB, et al. Regulation of mesangial cell hexokinase activity and expression by heparin-binding epidermal growth factor-like growth factor: epidermal growth factors and phorbol esters increase glucose metabolism via a common mechanism involving classic mitogen-activated protein kinase pathway activation and induction of hexokinase II expression. J. Biol. Chem. 277: 14370-14378, 2002. PubMed: 11782486 61224: Maile S, et al. The morphology of mesangial cells cultured at high density and in collagen gels. Histol. Histopathol. 15: 403-414, 2000. PubMed: 10809358 61225: Coy PE, et al. LPA is a novel lipid regulator of mesangial cell hexokinase activity and HKII isoform expression. Am. J. Physiol. Renal Physiol. 283: F271-F279, 2002. PubMed: 12110510 90657: Taneja N, et al. Proinflammatory interleukin-1 cytokines increase mesangial cell hexokinase activity and hexokinase II isoform abundance. Am. J. Physiol. Cell Physiol. 287: C548-C557, 2004. PubMed: 15070811