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免疫原 :GAPDH fusion protein Ag0766
亚型 :IgG2b
形态 :Liquid
保存条件 :Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
克隆性 :Monoclonal
标记物 :Unconjugated
适应物种 :arabidopsis, bombyx mori, bovine, branchiostoma belcheri, caenorhabditis elegans, canine, chicken, cho cell line, cynomorium songaricum, d. pulex, deer, duck, frog, fungus, goat, hamster, hermetia illucens, human, monkey, mouse, mussels, pig, plant
宿主 :Mouse
应用范围 :WB, IP, IHC, IF, FC, CoIP, ChIP
抗体英文名 :GAPDH Mouse Monoclonal Antibody
抗体名 : glyceraldehyde-3-phosphate dehydrogenase
规格 :1000UL/100UL/500UL
60004-1-Ig targets GAPDH in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat, yeast, plant, zebrafish samples.背景介绍
What is the molecular weight of GAPDH?
The molecular weight of GAPDH is 36 kD.
What is GAPDH?
GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic
production of ATP and pyruvate (PMID: 21895736).
What is the function of GAPDH?
GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 11035021).
Where in the cell is GAPDH localized?
GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus (PMIDs: 21895736, 12829261).
Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear functions?
Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is useful as a control for Western blots and RT-PCR.
What are the known protein-protein interactions of GAPDH?
GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).
Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.
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入驻年限:16年