产品详情
文献和实验
相关推荐
注册证号 ：/
英文名 ：GIBCO DPBS 培养基
CAS号 ：/
库存 ：5000
供应商 ：舜冉生物
肿瘤类型 ：/
细胞类型 ：/
ATCC Number ：/
品系 ：培养试剂
组织来源 ：/
相关疾病 ：/
物种来源 ：/
免疫类型 ：/
细胞形态 ：/
是否是肿瘤细胞 ：/
器官来源 ：/
运输方式 ：冷链运输
年限 ：5年
生长状态 ：/
规格 ：/
舜冉(上海)生物SERANA胎牛血清中国区总代理.舜冉(上海)生物所销售的血清，均为原装正品，一个月内出现质量问题，包退换！
血清的保存应该注意以下几点：
（1）切勿将血清在37℃放置太久,否则血清会变得浑浊,同时血清中的有效成分会破坏而影响血清质量.
需要长期保存的血清必须储存于-20℃ - 70℃ 低温冰箱中.4℃冰箱中保存时间切勿超过1个月.由于血清结冰时体积会增加约10%,因此,血清在冻入低温冰箱前,必须预留一定体积空间,否则易发生污染或玻璃瓶冻裂.
（2）瓶装血清解冻需采用逐步解冻法:-20℃ 至 -70℃ 低温冰箱中的血清放入4℃冰箱中溶解.然后移入室温,待全部溶解后再分装.在溶解过程中需不断轻轻摇晃均匀（小心勿造成气泡）,使温度与成分均一,减少沉淀的发生.切勿直接将血清从-20℃进入37℃解冻,这样因温度改变太大,容易造成蛋白质凝集而出现沉淀.
Gibco fetal bovine serum (FBS): frequently asked questions
What is the difference between fetal bovine serum and fetal calf serum?
There is no difference. Both terms refer to serum from an unborn cow.
How is dialyzed FBS processed?
It is dialyzed by tangential flow filtration against 0.15 M NaCl using a 10,000 molecular weight cutoff membrane until the glucose level is less than 5 mg/dL. Because this is not exhaustive dialysis, low molecular weight dialyzable components such as amino acids may not becompletely removed. Exhaustive dialysis is not performed because it can result in precipitation and inactivation of serum peptides.
What is the difference between Certified and Qualified FBS?
The serum is from the same source, collected and processed in the same way. The differences are in the testing performed on the serum and the specifications for release. Certified serum undergoes additional biochemical and hormonal testing. Certified FBS has a lower concentration of endotoxin (5 EU/mL or less) and hemoglobin (15 mg/dL or less).What does International Serum Industry Association (ISIA) traceability certification mean?
It means the company maintains all auditable history of quality and quantity of materials from the point of collection (including specific animal numbers for donor products), and documentation supporting all stages of processing and transportation or commercial transactions.How do I heat-inactivate serum?
Heat it at 56°C in a water bath for 30 minutes and swirl the bottle every 10 minutes or so. For accuracy, use a second bottle of similar size as a control and add an equivalent volume of water to the control bottle. Place a thermometer in the control bottle to see when 56°C is reached. Set your timer for 30 minutes at this point.Why would I heat-inactivate serum?
Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.What is the importance of gamma-irradiated sera?
Gamma irradiation is recognized as an effective method for inactivating viruses in animal-origin material. Based on
USDA regulations for the general requirements for antibody products (9CFR, Section 113.450), the minimum dosage for treating blood derivatives of animal origin is 25 kGy (kilogray). Certain European countries require products
to be treated prior to importation with a minimum dose of 25 kGy.
We will gamma-irradiate serum on request. We have validated a process for using gamma irradiation in the range of 30–45 kGy to inactivate the most common bovine viruses and mycoplasmas that may be present in FBS. The level of inactivation is 6–8 logs for viruses and 6–7 logs
for mycoplasmas. We have also demonstrated that physiochemical properties and cell culture performance of serum are not altered by gamma irradiation at
levels of 30–40 kGy.
Can I get custom serum products for my specific research requirements?
Yes, we offer a wide range of custom options. Please contact our serum specialists to discuss your application and research needs. We will work with you to supply sera that is specially processed and tested to meet your individual requirements. Some examples of our custom capabilities are heat inactivation, gamma irradiation, and testing for tetracycline residues.What is the shelf life of FBS?
Most FBS products that are unopened and stored in a freezer have a 5-year shelf life. Also, you can refer to the expiration date on the bottle and the Certificate of Analysis for this information.Why does material precipitate in FBS upon storage in the refrigerator?
Gibco™ FBS is not pre-aged. When stored at 2–8°C, various proteins and lipoproteins in the serum (e.g., cold agglutinins, fibrinogen, vitronectin) may aggregate and form visible material or turbidity; however, this should not affect serum performance. We recommend that you store FBS at–20°C and avoid repeated freeze-thaw cycles.
My FBS contains flocculence. What could it be?
Flocculence may appear in FBS for a variety of reasons. The most common reason is the denaturation of serum lipoproteins. You may observe fibrin, a clot-forming protein, after the serum has been thawed. This should not affect serum performance.
To remove flocculence, transfer the serum to sterile tubes and centrifuge the material briefly at 400 x g, then filter the resulting supernatant along with your media. Do not attempt to filter serum containing flocculence, as it may clog filters.
Is serum sterile?
Fetal bovine serum is triple-filtered through 0.1 µm filters. Bovine serum is filtered through 0.2 µm filters.Is serum tetracycline-free?
Our limit of detection for tetracycline is 19.7 ng/mL, and tetracycline must be “undetectable” to pass inspection, but we cannot say that it is tetracycline-free.How should serum be thawed?
Serum should be thawed overnight at 2–8°C.If my FBS arrives partially thawed, can I still use it?
All Gibco FBS is shipped frozen and packaged in dry ice, so it should arrive frozen. You can still confidently use FBS that is partially thawed, if at least two-thirds of it is frozen.
Can I aliquot and refreeze serum?
Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.How long can I store thawed serum at 2–8°C?
Serum should be used within 2–4 weeks when stored at 2–8°C.
How can I minimize lot-to-lot variability?
You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco™ iMatch™ Sera Lot Matching Tool.What sizes of packaging are available?
Gibco FBS is available in 50 mL, 100 mL, 500 mL, and 1,000 mL bottles.How can I sample FBS or set up a reserve?
Your serum sales representative will be happy to assist you in getting a reserve set up for a specific lot or testing to see what lot may work best for you.
Alternatively, you can request a FBS sample yourself at
thermofisher.com/fbssamplerequest.
产品验收：
（1） 血清内外包装均应完好，无破损、裂缝、溢渗等现象；
（2）血清到货时应为冷冻或冰水混合状态，不应完全融化；
（3）若出现上述现象，请拍照取证后及时与我们联系，以便进行更换。
x"> 舜冉(上海)生物SERANA胎牛血清中国区总代理.
舜冉(上海)生物所销售的血清，均为原装正品，一个月内出现质量问题，包退换！
血清的保存应该注意以下几点：
（1）切勿将血清在37℃放置太久,否则血清会变得浑浊,同时血清中的有效成分会破坏而影响血清质量.
需要长期保存的血清必须储存于-20℃ - 70℃ 低温冰箱中.4℃冰箱中保存时间切勿超过1个月.由于血清结冰时体积会增加约10%,因此,血清在冻入低温冰箱前,必须预留一定体积空间,否则易发生污染或玻璃瓶冻裂.
（2）瓶装血清解冻需采用逐步解冻法:-20℃ 至 -70℃ 低温冰箱中的血清放入4℃冰箱中溶解.然后移入室温,待全部溶解后再分装.在溶解过程中需不断轻轻摇晃均匀（小心勿造成气泡）,使温度与成分均一,减少沉淀的发生.切勿直接将血清从-20℃进入37℃解冻,这样因温度改变太大,容易造成蛋白质凝集而出现沉淀.
Gibco fetal bovine serum (FBS): frequently asked questions
What is the difference between fetal bovine serum and fetal calf serum?
There is no difference. Both terms refer to serum from an unborn cow.
How is dialyzed FBS processed?
It is dialyzed by tangential flow filtration against 0.15 M NaCl using a 10,000 molecular weight cutoff membrane until the glucose level is less than 5 mg/dL. Because this is not exhaustive dialysis, low molecular weight dialyzable components such as amino acids may not becompletely removed. Exhaustive dialysis is not performed because it can result in precipitation and inactivation of serum peptides.
What is the difference between Certified and Qualified FBS?
The serum is from the same source, collected and processed in the same way. The differences are in the testing performed on the serum and the specifications for release. Certified serum undergoes additional biochemical and hormonal testing. Certified FBS has a lower concentration of endotoxin (5 EU/mL or less) and hemoglobin (15 mg/dL or less).What does International Serum Industry Association (ISIA) traceability certification mean?
It means the company maintains all auditable history of quality and quantity of materials from the point of collection (including specific animal numbers for donor products), and documentation supporting all stages of processing and transportation or commercial transactions.How do I heat-inactivate serum?
Heat it at 56°C in a water bath for 30 minutes and swirl the bottle every 10 minutes or so. For accuracy, use a second bottle of similar size as a control and add an equivalent volume of water to the control bottle. Place a thermometer in the control bottle to see when 56°C is reached. Set your timer for 30 minutes at this point.Why would I heat-inactivate serum?
Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.What is the importance of gamma-irradiated sera?
Gamma irradiation is recognized as an effective method for inactivating viruses in animal-origin material. Based on
USDA regulations for the general requirements for antibody products (9CFR, Section 113.450), the minimum dosage for treating blood derivatives of animal origin is 25 kGy (kilogray). Certain European countries require products
to be treated prior to importation with a minimum dose of 25 kGy.
We will gamma-irradiate serum on request. We have validated a process for using gamma irradiation in the range of 30–45 kGy to inactivate the most common bovine viruses and mycoplasmas that may be present in FBS. The level of inactivation is 6–8 logs for viruses and 6–7 logs
for mycoplasmas. We have also demonstrated that physiochemical properties and cell culture performance of serum are not altered by gamma irradiation at
levels of 30–40 kGy.
Can I get custom serum products for my specific research requirements?
Yes, we offer a wide range of custom options. Please contact our serum specialists to discuss your application and research needs. We will work with you to supply sera that is specially processed and tested to meet your individual requirements. Some examples of our custom capabilities are heat inactivation, gamma irradiation, and testing for tetracycline residues.What is the shelf life of FBS?
Most FBS products that are unopened and stored in a freezer have a 5-year shelf life. Also, you can refer to the expiration date on the bottle and the Certificate of Analysis for this information.Why does material precipitate in FBS upon storage in the refrigerator?
Gibco™ FBS is not pre-aged. When stored at 2–8°C, various proteins and lipoproteins in the serum (e.g., cold agglutinins, fibrinogen, vitronectin) may aggregate and form visible material or turbidity; however, this should not affect serum performance. We recommend that you store FBS at–20°C and avoid repeated freeze-thaw cycles.
My FBS contains flocculence. What could it be?
Flocculence may appear in FBS for a variety of reasons. The most common reason is the denaturation of serum lipoproteins. You may observe fibrin, a clot-forming protein, after the serum has been thawed. This should not affect serum performance.
舜冉（上海）生物科技有限公司
实名认证
金牌会员
入驻年限：7年
