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库存 :75
英文名 :Psp124BI
保质期 :长期
供应商 :北京百奥莱博科技有限公司
保存条件 :-20℃
规格 :1kU
特别提示:包括Psp124BI内切酶在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:Psp124BI内切酶
英文名称:Psp124BI
产品货号:GS2113
产品规格:1kU
储存条件:冷冻(-20℃)
技术规格
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(HindIII-digest)in 1hourat 37℃ in a total reaction volume of 50μl. |
Recognition sites | GAGCT↑C |
来源 | Pseudomonas species 124B |
Assayed on | Lambda DNA(HindIII-digest) |
Optimal buffer | G?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.) |
Optimal temperature | 37℃ |
Storage conditions | 10mMTris-HCl(pH 7.5);250 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100 μg/ml BSA;50%glycerol;Store at -20℃. |
Ligations | After 20-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃. |
Methlation effect | Methylation sensitivity not tested |
Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
Campatible Ends | Ecl136II^,Eco53kI^,EcoICRI^,SacI,SstI |
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