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保存条件 :-20°C, sealed storage, away from moisture and light
库存 :货期:1-2天
供应商 :MedChemExpress LLC
CAS号 :273221-67-3
规格 :100 μg
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Fluo-4 AM
CAS No. : 273221-67-3
MCE 国际站:Fluo-4 AM
产品活性:Fluo-4 AM 是一种常用的检测细胞内 Ca2+ 浓度的探针。
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright.?Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
?1.?Count the cells and take 106 cells from each sample (control and experiment/s).
?2.?Collect the cells (5 min, 3000×g, 4 °C) and wash once in PBS.
?3.?Resuspend the cells in 0.5-ml PBS and add 0.5 μl of Fluo-4-AM (1 mM stock) to a final concentration of 1 μM. Incubate at 37 °C for 1 h.
?4.?Wash the cells three times (2 min, 3000×g) with PBS and finally resuspend in 1-ml PBS. Separate into 2 flow cytometry tubes—0.5 ml in each.
?5.?Evaluate the staining by flow cytometry and analyze the data by a software such as CellQuest software.
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