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供应商 :复祥生物
细胞类型 :普通细胞株-科研实验
ATCC Number :HTB-1
物种来源 :人
运输方式 :常温
生长状态 :正常
库存 :大量
J82 人膀胱移行细胞癌MEM培养基(GIBCO)+10%胎牛血清。细胞货期8-10个工作日
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Designations: | J82 人膀胱移行细胞癌 | ||
Biosafety Level: | 1 | ||
Shipped: | frozen | ||
Medium & Serum: | See Propagation | ||
Growth Properties: | adherent | ||
Organism: | Homo sapiens | ||
Morphology: | epithelial |
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Source: | Organ: urinary bladder Disease: transitional cell carcinoma |
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Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
Tumorigenic: | Yes | ||
Antigen Expression: | HLA A2, Aw32, B5, B12, Cw5; Blood Type A | ||
DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 10,12 D16S539: 11,12 D5S818: 12,13 D7S820: 9,11 THO1: 9.3 TPOX: 11,12 vWA: 17,18 |
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Cytogenetic Analysis: | The cell line is aneuploid human male (XY), with most chromosome counts in the triploid range. However, the chromosome count range is quite broad, extending from hyperdiploid to hexaploid. Normal chromosomes N11 and N20 are under-represented with respect to the other normal chromosomes: altered forms of these two chromosomes are prominent as marker chromosomes. Chromosome N13 tends towards over-representation. Five marker chromosomes are noted: 20q+, 11q+, 8p+, del(1)(q31), 5p+(HSR). One of these, 20q, is identical to marker chromosome M1 of C. O'Toole, et al., Br. J. Cancer 38: 64, 1978. The remainder are not as clearly related to the original marker chromosomes noted by those authors. | ||
Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1-2 PGM1, 1 PGM3, 2 |
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Age: | 58 years | ||
Gender: | male | ||
Ethnicity: | Caucasian, Swedish | ||
Comments: | Electron microscopic examination did not reveal desmosomes but varying amounts of rough endoplasmic reticulum and prominent microfilaments were observed. Contains the ras (H-ras) oncogene. |
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Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended Medium Renewal: 2 to 3 times per week |
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Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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References: | 21849: O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125. 22325: O'Toole C, et al. Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma. Br. J. Cancer 38: 64-76, 1978. PubMed: 687519 24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 32266: Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064 |
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