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CAS号 : 010
注册证号 :0000
英文名 :Human Epidermal Keratinocytes-adult
物种来源 :human
相关疾病 :否
组织来源 :皮肤组织
品系 :原代细胞
ATCC Number :否
细胞类型 :原代细胞
肿瘤类型 :否
免疫类型 :否
细胞形态 :咨询销售
生长状态 :贴壁生长
年限 :5-10年
运输方式 :干冰
器官来源 :Human
是否是肿瘤细胞 :不是
库存 :大量
供应商 :中乔新舟
规格 :5 x 10^5 cells/vial
Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应
美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21
种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。
确保了实验结果的重复性和连贯性。
请登录Sciencell公司官方网站(www.zqxzbio.com 或www.sciencellonline.com )以确保购买正规公司产品
人表皮角质细胞--成人说明:
货号:2110
角质层是复扁平上皮细胞。角质细胞因含有大量的角蛋白而得名,在表皮基底层,角质细胞分化、增殖,随着角质细胞
产生角蛋白并上移至表皮表面后,发生程序性死亡。角蛋白细胞增殖、分化及凋亡是复杂且严格控制的过程。角蛋白
细胞可产生很多细胞因子和生长因子,包括IL- 1, IL-3, IL-6, IL-8, 集落刺激因子, TNF-alpha, TGF-alpha and beta, 成
纤维细胞生长因子, 双调蛋白和PDGF。除了保护功能之外,角蛋白细胞在组织稳态、伤口恢复、癌症以及皮肤基因治
疗上有着重要的作用。人角蛋白细胞能表达粘附分子和细胞因子,这说明了它能参与皮肤免疫和动态平衡。
<2100>人表皮角质细胞(HEK-n)-新生和<2110>人表皮角质细胞(HEK-a)-成人分别提取于新生儿和成人的表皮组
织,于原代冻存。每管含有细胞数>5×105cells/ml,此细胞通过Cytokeratine-8, -18 and -19免疫荧光染色验证,经测
试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。
推荐培养基:(KM, Cat. No. 2101)
DISCRIPTION
The epithelial layer of the skin provides an essential function as a protective barrier against insult from the
outside environment. The major cell type in this layer is keratinocytes which consists around 85% of living
epidermal cells. Kertinocytes are the cells of stratified squamous epithelia. It is so named because its most
abundant protein is keratin. Keratinocyte divide and are thus generated in the basal layer of the epidermis; this
division is followed by a programmed death as they produce keratin and move up to the surface of the epidermis.
Keratinocyte proliferation, differentiation, and apoptosis are a complex and carefully choreographed process [1].
Keratinocytes have been demonstrated to produce a wide range of cytokines and growth factors, including
IL- 1, IL-3, IL-6, IL-8, colony-stimulating factors, TNF-alpha, TGF-alpha and beta, fibroblast growth factor,
amphiregulin and PDGF [2, 3]. Besides the protective function, keratinocyte also plays a central role in tissue
homeostasis, wound healing, cancers, and skin-based gene-therapy. Human keratinocytes express adhesion
molecules and cytokines indicating their participation in skin innate immunity and homeostasis in vivo [4, 5].
HEK-a from ScienCell Research Laboratories are isolated from human adult epidermis. HEK-a are cryopreserved on
primary culture and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HEK-a are characterized by
immunofluorescent method with antibodies to cytokeratine-8, -18 and -19. HEK-a are negative for HIV-1,
HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEK-a are guaranteed to further expand for 15 population
doublings in the conditions provided by ScienCell Research Laboratories.
Reference
[1] Eckert, R. L., Efimova, T., Dashti, S. R., Balasubramanian, S., Deucher, A., Crish, J. F., Sturniolo, M. and Bone, F. (2004)
Keratinocyte survival, differentiation, and death: many roads lead to mitogen-activated protein kinase. J Investig
Dermatol Symp Proc 7(1):36-40.
[2] Luger, T. A. (1992) Epidermal cytokines and growth factors. Marcel Dekker, Inc., New York , pp. 147-162.
[3] John C. Ansel, Jay P. Tiesman, John E. Olerud, James G. Krueger, Jeffrey F. Krane,t David C. Tara, Gary D. Shipley,
Debra Gilbertson, Marcia L. Usui,I and Charles E. Hart (1993) Human Keratinocytes Are a Major Source of Cutaneous
Platelet-derived Growth Factor. J. Clin. Invest. 92:671-678.
[4] Song, P. I., Park, Y. M., Abraham, T., Harten, B., Zivony, A., Neparidze, N., Armstrong, C. A. and Ansel, J. C. (2004)
Human keratinocytes express functional CD14 and toll-like receptor 4. J Invest Dermatol 119(2):424-32.
[5] de Panfilis, G., Semenza, D., Lavazza, A., Mulder, A. A., Mommaas, A. M. and Pasolini, G. (2004) Keratinocytes
constitutively express the CD95 ligand molecule on the plasma membrane: an in situ immunoelectron microscopy
study on ultracryosections of normal human skin. Br J Dermatol. 147(1):7-12.
Sciencell部分产品目录:
真皮细胞系统 | |||
中文名 | 货号 | 简称 | 英文名称 |
人真皮微血管内皮细胞 | 2000 | HDMEC | Human Dermal Microvascular Endothelial Cells |
人真皮淋巴内皮细胞 | 2010 | HDLEC | Human Dermal Lymphatic Endothelial Cells |
人真皮微血管内皮细胞-成人 | 2020 | HDMEC-a | Human Dermal Microvascular Endothelial Cells-adult |
人表皮角质形成细胞-新生儿 | 2100 | HEK | Human Epidermal Keratinocytes-neonatal |
人表皮角质形成细胞- 成人 | 2110 | HEK-a | Human Epidermal Keratinocytes-adult |
人表皮角质形成细胞- 胎儿 | 2120 | HEK-f | Human Epidermal Keratinocytes-fetal |
人表皮黑色素细胞--浅色素 | 2200 | HEM-l | Human Epidermal Melanocytes-light |
人表皮黑色素细胞--中色素 | 2210 | HEM-m | Human Epidermal Melanocytes-medium |
人表皮黑色素细胞--深色素 | 2220 | HEM-d | Human Epidermal Melanocytes-dark |
人表皮黑素细胞-成人 | 2230 | HEM-a | Human Epidermal Melanocyte-adult |
人真皮成纤维细胞-胎儿 | 2300 | HDF-f | Human Dermal Fibroblasts-fetal |
人真皮成纤维细胞- 新生儿 | 2310 | HDF-n | Human Dermal Fibroblasts-neonatal |
人真皮成纤维细胞- 成人 | 2320 | HDF-a | Human Dermal Fibroblasts-adult |
人真皮成纤维细胞-胎 儿-丝裂霉素处理 | 2350 | HDF-f-mt | Human Dermal Fibroblasts-Fetal-Mitomycin C treated |
大鼠细胞系统 | |||
中文名 | 货号 | 简称 | 英文名称 |
大鼠表皮角质形成细胞-新生大鼠 | R2100 | REK-n | Rat Epidermal Keratinocytes-neonate |
大鼠真皮成纤维细胞 | R2300 | RDF-n | Rat Dermal Fibroblasts |
大鼠真皮成纤维细胞- 成人 | R2320 | RDF-a | Rat Dermal Fibroblasts-adult |
热烈祝贺上海中乔新舟生物科技有限公司成为美国Sciencell公司在中国正规一级代理商: Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应 Sciencell公司部分原代细胞目录(如需要其他细胞资料请发邮件至 wwwfudan@163.com索取):
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