BD cornning 354248 354230 354234 356230 356231 356234 356235 356237

规格 ：10ml

BD全线特约代理  常用基质等 备货充足 价格优势大  授权代理 超低折扣
BD产品货号：354230 354234 356230 356231 356234 356235 356237

BD Matrigel基质胶/基质膜
操作指南
BD采用专利技术，从富含胞外基质蛋白的EHS小鼠肿瘤中分离出BD Matrigel 基底膜基质，其主要成分由层粘连蛋白，Ⅳ型胶原，巢蛋白，硫酸肝素糖蛋白等组成，还包含生长因子和基质金属蛋白酶等。BD Matrigel基底膜基质在室温条件下，聚合形成具有生物学活性的三维基质，模拟体内细胞基底膜的结构、组成、物理特性和功能，有利于体外细胞的培养和分化，以及对细胞形态、生化功能、迁移、侵染和基因表达的研究。
BD Matrigel 基底膜基质形成的三维培养基质，可促进上皮细胞、肝细胞、Sertoli细胞、黑色素瘤细胞、血管内皮细胞、甲状腺细胞及毛囊细胞等的贴壁与分化。同时，Matrigel还能影响乳腺上皮细胞的蛋白表达，支持外周神经的新生和牛输卵管上皮细胞的分化。高浓度的Matrigel适用于研究体内血管生成和肿瘤细胞迁移及肿瘤模型的建立等。
BD Matrigel基质胶/基质膜
产品特性
Matrigel基质会有色差变化（淡黄色到深红色），是由于酚红和碳酸氢盐与CO2作用引起的，但是与5％CO2平衡后色差即会减少。冻融后，轻轻摇晃试剂瓶使Matrigel分散均匀。所有操作均需在无菌环境下进行，试剂瓶瓶盖可用70％乙醇擦拭，并自然干燥。应使用预冷的移液器以保证Matrigel呈匀浆状。
细胞可在0.5mm厚度的Matrigel基质层表面生长，也可在1mm厚度的Matrigel三维基质内生长。过度稀释的Matrigel会形成非胶质的蛋白层，可以用于细胞贴壁，但不能用于细胞的分化研究。
注意：可将冻融后的Matrigel分装在多个小管，所有分装均需用预冷的冻存管，迅速冷冻并保存，避免多次冻融。
Matrigel在22-35℃温度环境下快速成胶，因此溶解时在4℃冰上过夜冻融（4度时会随着温度的上升部分成胶）。所有用品在使用前需置于冰浴，必须使用预冷的移液管、吸头及小管操作Matrigel。成胶后的Matrigel可以在4℃24－48小时后重新呈液态。
推荐包被与成胶方法
注意：为了保证Matrigel基质膜的成胶性能与稳定性，稀释浓度不应低于1：3，可用无血清培养基稀释，Matrigel成胶后立即使用。
薄胶成胶方法：
  1.冻融后，用预冷的移液枪头混匀Matrigel基质成匀浆状。
  2.将需要使用的培养板置于冰上，加入浓度为50μL/cm2生长面积的Matrigel基质。
  3.在37℃放置30分钟，即可使用。
厚胶成胶方法：
  1.冻融后，用预冷的移液枪头混匀Matrigel基质成匀浆状。
  2.将需要使用的培养板置于冰浴，将培养的细胞与Matrigel基质混合，用移液枪头使其悬浮于基质中。加入浓度为150－200μL/cm2生长面积的Matrigel基质。
  3.在37℃放置30分钟，可成胶。可以加入细胞培养的基质，也可使细胞直接生长在胶表面。
薄层包被方法：
 1.冻融后，用预冷的移液枪头混匀Matrigel基质成匀浆状。
 2.根据使用需要，采用无血清培养基稀释Matrigel基质。根据实验需要确定最佳包被浓度。
 3.将稀释的Matrigel基质包被于所需的培养器皿中，包被量至少覆盖整个器皿的生长表面。室温下孵育1小时。
  4.去除未结合的Matrigel，用无血清培养基轻轻地冲洗。
351130 Falcon 96 Well Multiwell Insert System with 1.0 µm Pore Translucent Polyester (PET) Membrane, with Feeder Tray and Lid, 1/Case
351131 Falcon 96 Well Multiwell Insert System with 1.0 µm Pore Translucent Polyester (PET) Membrane, with Feeder Tray and Lid, 5/Case
351155 Corning FluoroBlok HTS 24 Well Multiwell Permeable Support System with 3.0µm High Density Polyester (PET) Membrane, Sterile, 1/Pk, 1/Cs
351156 Corning FluoroBlok HTS 24 Well Multiwell Permeable Support System with 3.0µm High Density Polyester (PET) Membrane, Sterile, 5/Pk, 5/Cs
351157 Corning FluoroBlok HTS 24 Well Multiwell Permeable Support System with 8.0µm High Density Polyester (PET) Membrane, Sterile, 1/Pk, 1/Cs
351158 Corning FluoroBlok HTS 24 Well Multiwell Permeable Support System with 8.0µm High Density Polyester (PET) Membrane, Sterile, 5/Pk, 5/Cs
351161 Corning FluoroBlok HTS 96 Well Multiwell Permeable Support System with 3.0µm High Density Polyester (PET) Membrane, Sterile, 1/Pk, 1/Cs
351162 Corning FluoroBlok HTS 96 Well Multiwell Permeable Support System with 3.0µm High Density Polyester (PET) Membrane, Sterile, 5/Pk, 5/Cs
351163 Corning FluoroBlok HTS 96 Well Multiwell Permeable Support System with 8.0µm High Density Polyester (PET) Membrane, Sterile, 1/Pk, 1/Cs
351164 Corning FluoroBlok HTS 96 Well Multiwell Permeable Support System with 8.0µm High Density Polyester (PET) Membrane, Sterile, 5/Pk, 5/Cs
351181 Falcon 24 Well Multiwell Insert System with 1.0 µm Pore High Density Polyester (PET) Membrane, 5/Case
351183 Falcon HTS 24 Well Multiwell Permeable Support System with 3.0µm High Density Polyester (PET) Membrane, Sterile, 1/Pack, 5/Case
351184 Falcon HTS 24 Well Multiwell Permeable Support System with 8.0µm High Density Polyester (PET) Membrane, Sterile, 1/Pack, 1/Case
351185 Falcon HTS 24 Well Multiwell Permeable Support System with 8.0µm High Density Polyester (PET) Membrane, Sterile, 1/Pack, 5/Case
351186 Falcon 24 Well Polystyrene Feeder Tray, with Lid, Sterile, 5/Pack, 5/Case
353015 Corning Gentest Pre-coated PAMPA Plate System, with Lid, 5/Case
353137 Corning CELLine Disposable Bioreactor
353924 Falcon 96 Well Feeder Polystyrene Tray, with Lid, Sterile, 5/Pack, 5/Case
353925 Falcon® 96 Square Well Angled Bottom Not Treated Multiwell Insert System, with Lid, Sterile, 5/Case
353928 Falcon 96 Square Well Flat Bottom Microplate, with Lid, Sterile, 5/Pack, 5/Case
353938 Falcon 96 Square Well Angled Bottom Insert Plate, with Lid, Sterile, 5/Case
354001 Corning® Epidermal Growth Factor (EGF), Mouse Natural (Culture Grade), 100µg, 1/Pack
354008 Corning® Fibronectin, Human, 1mg
354009 Corning® 7S Nerve Growth Factors (NGF), Mouse Natural, 100µg, 1/Pack
354010 Corning® Epidermal Growth Factor (EGF), Mouse Natural (Receptor Grade), 100µg, 1/Pack
354022 Corning BioCoat Poly-D-Lysine 1536 Well Black Flat Bottom Microplate, 5/Case
354037 Corning® Insulin-like Growth Factor (IGF-I), Human Recombinant (Culture Grade), 10µg, 1/Pack
354039 Corning® Transforming Growth Factor-b (TGF-b), Human Natural, 1µg, 1/Pack
354043 Corning® Interleukin-2 (IL-2), Human Recombinant, 10,000 BRMP Units, 1/Pack
354048 Corning® Granulocyte-Macrophage Colony Stimulating Factors (GM-CSF), Human Recombinant, 1µg, 1/Pack
354051 Corning® Platelet-Derived Growth Factor-BB (PDGF-BB), Human Recombinant, 10µg, 1/Pack
354052 Corning® Epidermal Growth Factor (EGF), Human Recombinant, 100µg, 1/Pack
354058 Corning® Interleukin-3 (IL-3), Mouse Recombinant, 10µg, 1/Pack
354060 Corning® Basic Fibroblast Growth Factors (bFGF), Human Recombinant, 10µg, 1/Pack
354066 Corning® Tumor Necrosis Factor-a (TNF-a), Human Recombinant, 10µg, 1/Pack
354068 Corning® Interleukin-4 (IL-4), Human Recombinant, 5µg, 1/Pack
354077 Corning® BioCoat™ Poly-D-Lysine 35mm Coverslip Bottom TC-Treated Dishes, 20/Pack, 20/Case, Nonsterile
354085 Corning® BioCoat™ Poly-L-Lysine 12 mm #1 German Glass Coverslip, 40/Pack, 80/Case
354086 Corning® Biocoat™ Poly-D-Lysine 12mm #1 German Glass Coverslip, Bulk Packed, 80/Case
354087 Corning® BioCoat™ Poly-D-Lysine/Laminin 12mm #1 German Glass Coverslip, Bulk Packed, 80/Case
354088 Corning® BioCoat™ Fibronectin 22mm Round #1 German Glass Coverslip, Bulk Packed, 60/Case
354089 Corning® BioCoat™ Collagen I 22mm Round  #1 German Glass Coverslip, Bulk Packed, 60/Case
354105 Corning® Stem Cell Factor (SCF), Human Recombinant, 10µg, 1/Pack
354107 Corning® Vascular Endothelial Growth Factor (VEGF), Human Recombinant, 10µg, 1/Pack
354115 Corning® T-Cell Culture Supplement with ConA (IL-2 Culture Supplement), Rat, 100mL, 1/Pack
354141 Corning BioCoat HTS 24 Well Multiwell Angiogenesis System for Endothelial Cell Invasion, 1/Pack, 1/Case
354142 Corning BioCoat HTS 24 Well Multiwell Angiogenesis System for Endothelial Cell Invasion, 1/Pack, 5/Case
354143 Corning BioCoat BioCoat Fibronectin  HTS 24 Well Multiwell Angiogenesis System with 3.0µm Membrane, 1/Case
354144 Corning BioCoat Fibronectin HTS 24 Well Multiwell Angiogenesis System with 3.0µm Membrane, 1/Pack, 5/Case
354147 Corning BioCoat Fibronectin HTS 96 Well Multiwell Angiogenesis System with 3.0µm Membrane, 1/Pack, 1/Case
354148 Corning BioCoat Fibronectin HTS 96 Well Multiwell Angiogenesis System with 3.0µm Membrane, 1/Pack, 5/Case
354149 Corning BioCoat Angiogenesis System: Endothelial Cell Tube Formation, 96 Well Black Multiwell Plate, 1/Case
354150 Corning BioCoat Angiogenesis System: Endothelial Cell Tube Formation, 96 Well Black Multiwell Plate, 5/Case
354151 Corning Human Umbilical Vein Endothelial Cells, HUVEC-2 Cells (>5x105 Cells), 1 Cryovial/Pack
354165 Corning BioCoat Matrigel Tumor Invasion FluoroBlok 24 Well Plate, with Lid, 1/Pack, 1/Case
354166 Corning BioCoat Matrigel Tumor Invasion FluoroBlok 24 Well Plate, with Lid, 1/Pack, 5/Case
354167 Corning BioCoat Matrigel Tumor Invasion FluoroBlok 96 Well Plate, with Lid, 1/Pack, 1/Case
354168 Corning BioCoat Matrigel Tumor Invasion FluoroBlok 96 Well Plate, with Lid, 1/Pack, 5/Case
354210 Corning® Poly-D-Lysine, 20mg
354216 Corning Calcein AM Fluorescent Dye, 500µg, 10x50µg
354217 Corning Calcein AM Fluorescent Dye, 1mg
354218 Corning DiIC12(3) Fluorescent Dye, 100mg
354221 Corning® rLaminin-521, Human, 100µg Vial
354222 Corning® rLaminin-521, Human, 1mg (10 x 100µg Vials)
354223 Corning® rLaminin-521, Human, 5mg (50 x 100µg Vials)
354230 Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, *LDEV-Free, 10mL
354232 Corning® Laminin, Mouse, 1mg
354233 Corning® Collagen IV, Mouse, 1mg
354234 Corning® Matrigel® Basement Membrane Matrix, *LDEV-Free, 10mL
354235 Corning® Dispase, 100mL
354236 Corning® Collagen I, Rat Tail, 100mg