| neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival, growth and differentiation of neurons.As proneurotrophins and mature neurotrophins elicit opposite biological effects,Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner.Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.
·Description
Recombinant Human precursor form of Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 2X229 amino acids as a homodimer and having a molecular mass of 52kDa.
·Quality Control
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Met-Lys.
Endotoxin: Less than 0.1ng/μg (1EU/μg) of hPro-BDNF as determined by LAL test.
·Formulation
Sterile and Lyophilized aliquots with a 0.2um filtered protein solution containing 20mM PBS.
·Reconstitution
It is recommended to reconstitute the lyophilized rHuPro-BDNF in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to working solutions.
·Storage
Lyophilized rHuPro-BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C, which is stable for up to twelve months from date of receipt. Upon reconstitution rHuPro-BDNF should be stored at 4°C between 2-7 days and aliquots of reconstituted samples are stable up to 3 months below -18°C for future use.
Please avoid freeze-thaw cycles. |
