人神经束膜细胞

Perineurial cells are of mesenchymal origin. They make up the perineurium, which has the
important role of maintaining the integrity of the internal peripheral nerve environment by
creating a physical barrier that, under physiologic condition, limits the entry of biologically active
proteins, infectious agents, and migration of blood-borne cells into the nerve bundles [1]. The
perineurial cells are characterized by distinct ultrastructural features, including non-branching thin
cytoplasmic processes coated by an external lamina and joined at their ends by a tight junction,
few organelles, actin and vimentin filaments, and numerous pinocytotic vesicles [2]. They initially
form a loose, permeable sheath around axons and Schwann cells, which may recruit them from the
surrounding mesenchyme, and from which they are separated by the extracellular matrix. These
cells later undergo a mesenchymal-to-epithelial transition, forming tight junctions and organizing
into the perineurium. The perineurial cells are immunoreactive for vimentin and epithelial membrane
antigen but not for the Schwann cell markers S100 protein [3].

HPNC from ScienCell Research Laboratories are isolated from human spinal nerves. HPNC are
cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume.
HPNC are characterized by immunofluorescent method with antibodies to Vimentin, S100, GFAP and
CD90. HPNC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPNC are
guaranteed to further expand for 15 population doublings in the conditions provided by ScienCell
Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HPNC in vitro.

Product Use

HPNC are for research use only. They are not approved for human or animal use, or for application
in in vitro diagnostic procedures.

Shipping

Dry ice.

Reference

[1] Salzer, J. L. (1999) Creating barriers: a new role for Schwann cells and desert hedgehog. Neuron
22:627629.
[2] Erlandson, R. A. (1991) The enigmatic perineurial cell and its participation in tumors and in tumor
like entities. Ultrastruct Pathol. 15:335-351.
[3] Ariza, A., Bilbao, J. M. and Rosai, J. (1988) Immunohistochemical detection of epithelial membrane
antigen in normal and perineurial cells and perineurioma. Am J Surg Pathol. 12:678-683.

Sciencell部分产品目录:

（三）小鼠正常细胞