Human NGF ELISA Kit (Sandwich ELISA) - LS-F36847

价格： $990

品牌：LSBio

货号：LS-F36847

产品详情

Human NGF ELISA Kit (Sandwich ELISA) - LS-F36847

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Size
Price
LS-F36847-1
1 plate
$990 USD
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Product Description

LS-F36847 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human NGF in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of NGF as low as 9.375 picograms per milliliter.

Specifications

Type

Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target

NGF
Reactivity

Human
Manual

Intended Sample Types

Plasma, Serum, Tissue Homogenates
Format

96-Well Strip Plate
Detection

Colorimetric - 450nm (TMB)
Measurement

Quantitative
Detection Range

15.6 - 1000 pg/ml
Sensitivity

9.375 pg/ml
Precision

Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage

Store at 4°C. Stable for 6 months.
Quality Assurance

Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components

User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers

Background

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NGF/NGFßantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- NGF/NGFß antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NGF/NGFß amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NGF/NGFß can be calculated.
Restrictions

For research use only.
Guarantee

This elisa kit carries the LSBio 100% Guarantee.
About NGF

Uniprot: P01138 NCBI: NM_002506 NP_002497.2