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免疫原 :Recombinant protein encompassing a sequence within the center region of human HSP90 beta. The exact sequence is proprietary.
亚型 :IgG
形态 :Liquid
保存条件 :Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性 :Polyclonal
标记物 :Unconjugated
适应物种 :Human, Mouse, Rat, Zebrafish, Drosophila
保质期 :12 months from the shipping date of the product.
抗原来源 :Human
目录编号 :GTX101423
级别 :Primary Antibodies
库存 :Available
供应商 :GeneTex
宿主 :Rabbit
应用范围 :WB, ICC/IF, IHC-P, IHC-Wm
浓度 :5.43 mg/ml (Please refer to the vial label for the specific concentration.)
靶点 :Hsp90 beta
抗体英文名 :Hsp90 beta antibody [N2C2], Internal
抗体名 :Hsp90 beta 抗体 [N2C2], Internal
规格 :100 μl/25 μl
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Hsp90 beta antibody [N2C2], Internal (GTX101423) at 1:300 dilution.
Hsp90 beta antibody [N2C2], Internal detects Hsp90 beta protein at cytosol on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: PFA-fixed zebrafish embryos.
Hsp90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:200.
Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX101423 diluted at 1:1000
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HSP90 beta antibody [N2C2], Internal detects HSP90 beta protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
HSP90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX101423 diluted at 1:2000
HSP90 beta antibody [N2C2], Internal detects HSP90AB1 protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HSP90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunofluorescence analysis of methanol-fixed HCT116, using HSP90 beta(GTX101423) antibody at 1:500 dilution.
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入驻年限:14年