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免疫原 ：Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys27 of human Histone H3. The exact sequence is proprietary.
亚型 ：IgG
形态 ：Liquid
保存条件 ：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
克隆性 ：Polyclonal
标记物 ：Unconjugated
适应物种 ：Human, Mouse, Rat, Zebrafish
保质期 ：12 months from the shipping date of the product.
抗原来源 ：Human
目录编号 ：GTX121184
级别 ：Primary Antibodies
库存 ：Available
供应商 ：GeneTex
宿主 ：Rabbit
应用范围 ：WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, Dot, EM
浓度 ：1 mg/ml(Please refer to the vial label for the specific concentration.)
靶点 ：Histone H3K27me3 (Tri-methyl Lys27)
抗体英文名 ：Histone H3K27me3 (Tri-methyl Lys27) antibody
抗体名 ：Histone H3K27me3 (Tri-methyl Lys27) 抗体
规格 ：100 μl/25 μl
Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H3K27me3 (Tri-methyl Lys27) antibody detects Histone H3K27me3 (Tri-methyl Lys27) protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein on zebrafish by whole mount immunohistochemical analysis. Sample: PFA-fixed 2 day-post-fertilization zebrafish embryo.Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) dilution: 1:100.
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histon H3 (tri-Methyl K27)(GTX121184) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H3K27me3 (Tri-methyl Lys27) antibody detects Histone H3K27me3 (Tri-methyl Lys27) protein at nucleus by immunofluorescent analysis.Sample: 293T cells were fixed in 4% PFA at RT for 15 min.Green: Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed A431, using Histone H3 (tri-Methyl K27)(GTX121184) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Histone H3K27me3 (Tri-methyl Lys27) antibody detects Histone H3K27me3 (Tri-methyl Lys27) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
U87 cells were grown under normoxic (DMEM 10% FBS, 16% O2), hypoxic (DMEM 10% FBS, 1% O2), and neurosphere conditions (DMEM/F12, B-27 supplement, growth factor (10ng/ml FGF and 20ng/ml EGF)). Cell lysate were Western blotted for H3K27me3 and Histone-H3 (loading control). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (Tri-methyl Lys27) antibody (GTX121184) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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入驻年限：14年
