产品详情
文献和实验
相关推荐
免疫原 ：Recombinant protein encompassing a sequence within the C-terminus region of human MCM4. The exact sequence is proprietary.
亚型 ：IgG
形态 ：Liquid
保存条件 ：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性 ：Polyclonal
标记物 ：Unconjugated
适应物种 ：Human, Mouse, Zebrafish
保质期 ：12 months from the shipping date of the product.
抗原来源 ：Human
目录编号 ：GTX109740
级别 ：Primary Antibodies
库存 ：Available
供应商 ：GeneTex
宿主 ：Rabbit
应用范围 ：WB, ICC/IF, IHC-P, IP, ChIP assay
浓度 ：1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点 ：IP/MS validation was supported by references (PMID:30377397)
抗体英文名 ：MCM4 antibody
抗体名 ：MCM4 抗体
规格 ：100 μl/25 μl
Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX109740 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using MCM4 antibody (GTX109740) at 1:300 dilution.
MCM4 antibody detects MCM4 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
MCM4 antibody (GTX109740) dilution: 1:5000
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM4 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody (GTX109740) diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using MCM4(GTX109740) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunofluorescence analysis of PFA-fixed HeLa, using MCM4(GTX109740) antibody at 1:500 dilution.
MCM4 antibody immunoprecipitates MCM4 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MCM4 protein by 4 μg MCM4 antibody (GTX109740)
7.5 % SDS-PAGE
The immunoprecipitated MCM4 protein was detected by MCM4 antibody (GTX109740) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
深圳欣博盛生物科技有限公司
品牌商实名认证
钻石会员
入驻年限：14年
