pET-11d大肠表达质粒价格_品牌:钦诚生物-丁香通官网

保存条件：负20度

保质期：2年

供应商：钦诚生物

规格：5ug

基本信息

启动子:	T7/lac
复制子:	ColE1 ori
终止子:	T7 terminator
质粒分类:	大肠杆菌载体；PET系列表达质粒
质粒大小:	5674bp
质粒标签:	N-T7
原核抗性:	氨苄青霉素Amp
克隆菌株:	DH5α
培养条件:	37℃，有氧，LB
表达宿主:	BL21(DE3)
培养条件:	37℃，有氧，LB
诱导方式:	IPTG或乳糖及其类似物
5'测序引物:	T7:TAATACGACTCACTATAGGG
3'测序引物:	T7-ter:TGCTAGTTATTGCTCAGCGG

质粒简介

pET-11a-d 载体具有N端T7标签和 BamH I 多克隆克隆位点。这些载体是很多pET家族载体的前身。pET-21a-d( )系列载体分别对应于 pET-11a-d 载体，但是包含有几个额外的特征序列。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。

LOCUS       pET11d    5674 bp     DNA circular   SYN
DEFINITION pET11d
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
COMMENT     ORIGDB|GenBank
COMMENT     VNTOAUTHORAD1|Novagen Inc.
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="pET11d"
                     /mol_type="other DNA"
     terminator      complement(188..316)
                     /label="T7_terminator"
     misc_feature    256..274
                     /label="T7_Terminal_primer"
     misc_feature    complement(326..358)
                     /label="T7_leader"
     misc_feature    complement(366..382)
                     /label="T7_transl_en_RBS"
     misc_feature    complement(400..427)
                     /label="lacO"
     promoter        complement(427..445)
                     /label="T7_promoter"
     misc_feature    complement(529..548)
                     /label="pBRrevBam_primer"
     CDS             582..1082
                     /label="ORF frame 3"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     misc_feature    823..1914
                     /label="lacI"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     CDS             955..1914
                     /label="ORF frame 1"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(1697..2392)
                     /label="ORF frame 1"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL
                     TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS
                     GPRWFAPAGENPV*"
     CDS             1970..2587
                     /label="ORF frame 2"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            1973..2587
                     /label="tet (576 - 1190)"
                     /gene="tet (576 - 1190)"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    3226..3417
                     /label="ROP"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    complement(3411..3433)
                     /label="pGEX_3_primer"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            complement(4606..5466)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     CDS             complement(4606..5466)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5508..5536)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
   61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
301 cgggctttgt tagcagccgg atccgaccca tttgctgtcc accagtcatg ctagccatgg
361 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
421 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
481 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
541 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
601 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
661 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
721 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt
781 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
841 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
901 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
961 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
1021 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
1081 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
1141 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
1201 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
1261 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
1321 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
1381 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
1441 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
1501 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
1561 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
1621 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
1681 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
1741 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
1801 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
1861 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
1921 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag
1981 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
2041 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
2101 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
2161 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
2221 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc
2281 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
2341 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
2401 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac cgctgatcgt
2461 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc
2521 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
2581 gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc
2641 aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc
2701 gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca
2761 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
2821 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
2881 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
2941 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
3001 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
3061 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
3121 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
3181 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
3241 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
3301 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
3361 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
3421 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
3481 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
3541 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
3601 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
3661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
3721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
3781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
3841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
3901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
3961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
4021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
4081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
4141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
4201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
4261 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
4321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
4381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
4441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
4501 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
4561 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
4621 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
4681 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
4741 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
4801 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
4861 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
4921 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
4981 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
5041 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
5101 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
5161 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
5221 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
5281 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
5341 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
5401 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
5461 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
5521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
5581 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
5641 taggcgtatc acgaggccct ttcgtcttca agaa