pLVX-Kdm1aT-r-IRES-Puro大鼠基因质粒

保存条件 ：负20度

保质期 ：2年

供应商 ：钦诚生物

规格 ：5ug

基本信息

启动子:	CMV promoter
复制子:	ori
终止子:	WPRE
质粒分类:	基因文库质粒；大鼠基因质粒；大鼠真核质粒
质粒大小:	10741bp
原核抗性:	氨苄青霉素Amp
筛选标记:	嘌呤霉素Puro
克隆菌株:	Stbl3等大肠杆菌
培养条件:	37℃，有氧，LB

质粒简介

             pLVX-Kdm1aT-r-IRES-Puro质粒是一个慢病毒哺乳细胞过表达质粒，CMV启动子驱动大鼠源Kdm1aT基因表达。

质粒图谱

质粒序列

LOCUS       Exported               10741 bp ds-DNA     circular SYN 15-JUN-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2803..5435
                     /label=Kdm1aT-r
     misc_feature    5444..6017
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6050..6649
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=Puro
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6663..7251
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7134..7145)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7458..8091
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8219..8235)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8243..8259
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8267..8297)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8312..8333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8621..9209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9380..10240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10241..10345)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10393..10527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcat gttgtctggg
     2821 aagaaggcgg cggcggcggc agcggcggcg gcggcagcag cggctgggac agaggccggg
     2881 cccggggcgg cgggcggcgc cgagaacggc tctgaggtgg ccgcgccgcc cgcgggcctg
     2941 gcgggccccg ccgacattgc tacgggggcg gcgggcgagc gcactccccg aaagaaggag
     3001 cctccgcggg cctcgccgcc cggaggcctg gccgagccgc ctgggtccgc cgggcctcag
     3061 gcggggccca cagccgggcc cggctccgct acacccatgg agacaggaat agccgagacc
     3121 ccggagggcc gacggaccag ccgacgcaag cgggcgaagg tagaatacag agaaatggat
     3181 gaaagcttgg ccaacctctc agaagatgaa tattattcag aagaagaaag aaatgccaaa
     3241 gcagagaagg agaagaagct tcccccgcca cctcctcaag ccccacctga ggaggaaaat
     3301 gaaagtgagc ccgaggagcc gtctgggcaa gcaggaggac ttcaagacga cagttctgga
     3361 gggtatggag acggccaagc atcaggtgtg gagggtgcag ctttccaaag cagacttccc
     3421 catgaccgaa tgacctctca ggaagcagcg tgtttcccag acatcatcag tgggcctcag
     3481 cagacacaga aggtttttct gttcatcagg aatcgcacat tgcagttatg gttggacaac
     3541 ccaaagattc agctgacatt tgaagctacg ctccagcagt tagaagcacc ttataacagt
     3601 gacactgtac ttgtccaccg agttcatagt tacttagagc gccacggtct tatcaacttc
     3661 ggcatctaca agaggataaa acctttacca attaaaaaga caggaaaggt gattattata
     3721 ggctcaggtg tttctggctt ggcagcagct cgacaactac agagttttgg gatggatgtc
     3781 acacttctgg aagccaggga tcgcgtaggt ggacgggtcg ctacatttcg aaaaggaaac
     3841 tatgtagctg atcttggagc catggtagta acaggtcttg gaggaaatcc catggctgtc
     3901 gtcagcaaac aagtaaatat ggaactggcc aagatcaagc aaaaatgccc actttatgaa
     3961 gccaatggac aagctgttcc aaaagagaaa gatgaaatgg tagaacaaga atttaaccgg
     4021 ttgcttgaag ccacctctta ccttagtcat cagttagact tcaacgtcct caataataaa
     4081 cctgtgtccc ttggccaggc gttggaggtt gtcattcagc tacaagaaaa gcacgtcaaa
     4141 gatgagcaga ttgaacactg gaagaagata gtgaaaactc aggaggaatt gaaagagctt
     4201 ctcaataaga tggtaaactt gaaggagaaa attaaagagc tccaccagca atacaaagaa
     4261 gcttcagaag tgaagccgcc cagagatatt acagctgagt tcctagtgaa aagcaagcac
     4321 agggacctga ctgccctctg caaggaatat gatgaattag ctgaaacaca aggaaagcta
     4381 gaagaaaaac ttcaagaatt ggaagccaat cccccaagtg atgtatatct ctcatcaaga
     4441 gacagacaaa tacttgactg gcattttgca aatcttgaat ttgccaacgc cacacctctc
     4501 tctaccctct ctcttaaaca ttgggatcag gatgatgact tcgagtttac tggcagccac
     4561 ctgacagtga ggaatggcta ctcgtgtgtg cctgtggctt tagctgaagg tttggacatt
     4621 aaactgaaca cagcagtgcg gcaggttcgc tacacagcct caggatgtga agtgattgct
     4681 gtgaacacac gctccacaag tcagaccttc atttataagt gtgacgcagt gctctgtacc
     4741 cttcctttgg gagtgctgaa gcagcagcca ccagctgttc agtttgtgcc gcctcttcct
     4801 gagtggaaaa catccgcagt ccaaaggatg ggatttggca accttaacaa ggtggtgcta
     4861 tgttttgacc gtgtgttctg ggatccaagt gtcaatttgt ttgggcacgt tggcagtaca
     4921 actgctagca ggggcgagct cttcctcttc tggaacttat ataaagctcc aatactcttg
     4981 gctctggtag caggcgaagc tgctggcatt atggagaaca ttagtgatga tgtgattgtc
     5041 ggccggtgcc tggccattct caaagggatt tttggcagca gtgcagtacc acagcccaag
     5101 gaaactgtgg tgtctcgttg gcgtgctgat ccctgggccc ggggctccta ttcttatgtg
     5161 gctgcaggat cttctggaaa tgactatgat ttaatggctc agccaatcac tcctggtccc
     5221 tcaattccag gtgccccaca gccaattcca agactcttct ttgctggaga acatacaatc
     5281 cgcaactacc cagctacggt ccatggtgct ctgctgagtg ggcttcgaga agcaggaagg
     5341 attgccgacc agtttttggg agccatgtac acgttgcctc gtcaggccac accgggtgtc
     5401 cctgcacagc agtccccaag tatgtgagcg gccgcggatc ccgcccctct ccctcccccc
     5461 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     5521 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     5581 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     5641 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     5701 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     5761 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     5821 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     5881 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     5941 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     6001 gaaaaacacg atgataagct tgccacaacc cacaaggaga cgaccttcca tgaccgagta
     6061 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     6121 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     6181 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6241 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6301 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6361 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6421 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6481 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6541 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6601 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaga cgcgtctgga
     6661 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6721 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6781 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6841 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6901 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6961 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7021 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     7081 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7141 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7201 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta
     7261 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     7321 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     7381 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7441 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     7501 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     7561 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     7621 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     7681 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     7741 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     7801 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     7861 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     7921 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7981 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8041 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     8101 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8161 ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg taatcatggt
     8221 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8281 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8341 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8401 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8461 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8521 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8581 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8641 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8701 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8761 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8821 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8881 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8941 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9001 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9061 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9121 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9181 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9241 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9301 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9361 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9421 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9481 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9541 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9601 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9661 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9721 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9781 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9841 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9901 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9961 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10021 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10081 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10141 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10201 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10261 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10321 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
    10381 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10441 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10501 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    10561 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    10621 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    10681 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    10741 t