文献支持Enzo中国授权代理商 Corticosterone ELISA kit/ADI-900-097

库存 ：大量

供应商 ：欣博盛生物

检测限 ：/

检测方法 ：竞争法ELISA

应用 ：/

适应物种 ：other

标记物 ：/

样本 ：血清，血浆等

规格 ：1*96wells

Enzo中国授权代理商 产品名称：Corticosterone ELISA kit 货号：ADI-900-097 规格：1*96wells

Enzo Life Sciences, Inc.隶属于全球上市公司Enzo Biochem，Inc.，总部设于美国纽约的法明代尔。Enzo在研发、生产、销售生物研究试剂方面拥有30多年的丰富成功经验，可提供多种细胞活性荧光染料、蛋白、抗体、多肽、小分子、标签探针染料及试剂盒等，用于研究目标的鉴定、高通量分析、基因表达分析、核酸检测、蛋白组学研究及细胞检测等。

更多详情请联系Enzo中国授权代理商欣博盛生物

Alternative size available: ADI-901-097 (5x96 wells)
Sensitive measurement of Corticosterone, detecting as little as 27 pg/ml
High throughput format with results in 3 hours for up to 39 samples in duplicate
Broad dynamic range
Species independent
Routinely used to monitor stress levels in zoo animals
The Corticosterone EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. The Corticosterone EIA kit has low cross-reactivity with related steroids. The broad dynamic range makes this kit ideal for a wide variety of sample matrices from any species.

Product Details
Sensitivity:    27.0 pg/ml (range 32 - 20,000 pg/ml)
 
Assay Time:    3 hours
 
Applications:    ELISA, Colorimetric detection
 
Application Notes:    For the quantitative determination of Corticosterone in culture supernatants, feces, plasma, serum, and saliva from any species. Cited sample types include cell lysate, urine and whole blood.
 
Wavelength:    405 nm
 
Species reactivity:    Species independent
 
Crossreactivity:    Corticosterone (100%), Deoxycorticosterone (21.3%), Desoxycorticosterone (21.0%), Progesterone (0.46%), Testosterone (0.31%), Tetrahydrocorticosterone (0.28%), Aldosterone (0.18%), Cortisol (0.046%) and <0.03%: Pregnenolone, Estradiol, Cortisone, 11-dehydrocorticosterone acetate
 
Shipping:    Blue Ice Not Frozen
 
Long Term Storage:    +4°C
 
Contents:    DxS IgG Microtiter plate, Assay buffer 15 concentrate, Steroid displacement reagent, Conjugate, Antibody, Wash buffer concentrate, Standard, pN-pp Substrate, Stop solution
 
Scientific Background:    Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Corticosterone production has been shown to increase with stress, with elevated corticosterone levels being associated with impairment of long term memory retrieval and following burn injury. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns.
 
Technical Info/Product Notes:    Cited samples:
Hormone ELISAs: Cited Samples
Wildlife Endocrinology: Cited Sample Types
 
Protocol:    Suggested Protocol for Small Volumes of Serum/Plasma Samples
Let all solutions come to room temp before use.
Aliquot 10µl of each sample into microfuge tube.
Make 1ml 1:100 Steroid Displacement Reagent (SDR) solution (immediately before use) in deionized water or PBS (not assay buffer)
Add 10µl 1:100 SDR to each sample tube.
Vortex, let stand >5 minutes before diluting with EIA buffer.
Add 380µl EIA assay buffer to each plasma tube, vortex.
Final dilution is ~1:40 (This final dilution may not be appropriate for every sample; thus, optimal dilution must be determined by each end user for their experimental sample group.)
 
Regulatory Status:    RUO - Research Use Only