Signal-Seeker™ Ubiquitin Enrichment Kit (30 assays)

保存条件 ：参照说明书

英文名 ：Signal-Seeker™ Ubiquitin Enrichment Kit (30 assays)

库存 ：1

供应商 ：北京博蕾德生物科技有限公司

规格 ：KIT30assays

Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、蛋白质转录后修饰（PTM）、细胞骨架研究相关的系列试剂盒和产品，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有产品的药物筛选服务。
The Signal-Seeker™ line of produts have been developed to allow simple analysis of key regulatory protein modifications by specialists and non-specialists alike. The comprehensive Signal-Seeker™ kits provide an affinity bead system to isolate and enrich modified proteins from any given cell or tissue lysate. The enriched protein population is then analyzed by standard western blot procedures using a primary antibody to the target protein. 

Product Uses Include

• Investigate transient regulatory mechanisms
• Measure signalling events of multiple pathway member proteins 
• Discover new modifications of your protein of interest
• Gain insight into regulatory mechanisms
• Measure endogenous or transiently expressed protein signalling events

• Kit contents

  The Ubiquitination kit contains the following components:

  Lysis and protein quantitation step	IP and pre-clear step	Wash step	Elution step	Western step
  BlastR™ Lysis Buffer   BlastR™ Dilution Buffer  BlastR™ Filters  Protease Inhibitor Cocktail  De-Ubiquitinase inhibitor  Cocktail  Precision Red™ Protein  Assay Reagent	Ubiquitination Affinity  Beads  Ubiquitination Control  Beads	BlastR™ Wash  Buffer	Spin Columns  Bead Elution  Buffer	Chemiluminescent  Reagent A  Chemiluminescent  Reagent B  Anti-ubiquitin-HRP  antibody

   

  Example results

  There are many applications for these kits (see next section below), here we describe an interesting application for Rac1 protein: