CRL-7869 Hs 738.St/Int 人成纤维细胞

供应商 ：ATCC

ATCC Number ：CRL-7869

品系 ：查看相关资料

组织来源 ：人成纤维细胞

相关疾病 ：查看相关资料

物种来源 ：人

免疫类型 ：查看相关资料

器官来源 ：人

运输方式 ：常温

年限 ：2020

生长状态 ：正常

库存 ：大量

是否是肿瘤细胞 ：否

CRL-7869 Hs 738.St/Int 人成纤维细胞

ATCC® Number:	CRL-7869™	Price:	$429.00
Designations: Hs 738.St/Int Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Homo sapiens (human) Morphology: fibroblast Source: Organ: mixed; stomach; intestine Disease: normal Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. DNA Profile (STR): Amelogenin: X,Y CSF1PO: 10,11 D13S317: 8,11 D16S539: 9,11 D5S818: 11,13 D7S820: 8,12 THO1: 7,9.3 TPOX: 8 vWA: 16,18 Cytogenetic Analysis: modal number = 46; range = 44 to 92 Age: 18 weeks gestation fetus Gender: male Ethnicity: Caucasian Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. Please see the NBL Repository description. Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Every 2 to 3 days Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor termperature Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020

ATCC® Number: CRL-7869™ Price: $429.00
Designations: Hs 738.St/Int
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: fibroblast


Source: Organ: mixed; stomach; intestine
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,11
D16S539: 9,11
D5S818: 11,13
D7S820: 8,12
THO1: 7,9.3
TPOX: 8
vWA: 16,18
Cytogenetic Analysis: modal number = 46; range = 44 to 92
Age: 18 weeks gestation fetus
Gender: male
Ethnicity: Caucasian
Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor termperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002