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样本 :血清/血浆/组织匀浆液
库存 :1000
适应物种 :Mouse
应用 :科研使用
检测方法 :ELISA
检测限 :1.563 - 100 pg/ml
供应商 :康朗生物
规格 :96T
Mouse IL6R / IL6 Receptor ELISA Kit (Sandwich ELISA)
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LS-F40366-1
1 plate
$740 USD
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5% off 5+ units
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Product Description
LS-F40366 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse IL6R / IL6 Receptor in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of IL6R / IL6 Receptor as low as 0.938 picograms per milliliter.Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IL6R / IL6 Receptor
Reactivity
Mouse
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
1.563 - 100 pg/ml
Sensitivity
0.938
Precision
Intra-Assay: CV%<10% Inter-Assay: CV%<10%
Storage
Store at 4°C. Do not freeze.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Protocol
- Prepare all reagents, samples and standards. Add 100 ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and add 100 ul prepared Detection Antibody and incubate of 1 hour at 37°C. Aspirate and wash 3 times. Add 100 ul HRP Conjugate and incubate for 30 minutes at 37°C. Aspirate and wash 5 times. Add 90 ul Substrate Solution and incubate for 15-20 minutes at 37°C. Add 50 ul Stop Solution.Read immediately at 450nm.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
About IL6R / IL6 Receptor
P08887 NM_000565 NP_000556.1
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入驻年限:10年