CAS号 :无
库存 :大量
保存条件 :4℃密封避光
保质期 :1年
供应商 :普利莱
英文名 :Plant total protein extraction kit
规格 :50ml/100ml
植物总蛋白提取试剂 货号: P1258
适用范围:提取多种植物组织和细胞如苹果、花生、土豆、烟叶、菠菜、梅花等。
组成: 50mL或100mL无色透明试剂
储存:4ºC避光保存,12个月有效
以下列举部分使用普利莱植物蛋白提取试剂发表的SCI文章,供参考。
- Wang F X, Ma Y P, Yang C L, et al. Proteomic analysis of the sea‐island cotton roots infected by wilt pathogen Verticillium dahliae[J]. Proteomics, 2011, 11(22):
- 4296-4309. Yuan H, Meng D, Gu Z, et al. A novel gene, MdSSK1, as a component of the SCF complex rather than MdSBP1 can mediate the ubiquitination of S-RNase in apple[J]. Journal of experimental botany, 2014: eru164.
- Yu X, Jones H D, Ma Y, et al. (E)-β-Farnesene synthase genes affect aphid (Myzus persicae) infestation in tobacco (Nicotiana tabacum)[J]. Functional & integrative genomics, 2012, 12(1): 207-213.
- Wang F X, Ma Y P, Yang C L, et al. Proteomic analysis of the sea‐island cotton roots infected by wilt pathogen Verticillium dahliae[J]. Proteomics, 2011, 11(22): 4296-4309.
- Gao S, Yu B, Yuan L, et al. Production of transgenic sweetpotato plants resistant to stem nematodes using oryzacystatin-I gene[J]. Scientia Horticulturae, 2011, 128(4): 408-414.
描述:植物组织成分复杂含有较多的酚类物质、多糖、色素、次生代谢产物, 这使得植物蛋白质的分离提取变得困难。本试剂适用于多种植物根、茎、叶及果实等的新鲜或冻存组织,能够快速有效地提取植物中的可溶性和疏水性蛋白成分,有效去除组织中所含的多糖、脂质、此生代谢产物等干扰蛋白研究的成分,同时能够使提取的蛋白质处于最佳的活性状态和检测状态,适用于酶学活性测定,单向、双向蛋白电泳、Western Blot和免疫共沉淀等实验。
该产品被引用文献
- Wang F X, Ma Y P, Yang C L, et al. Proteomic analysis of the sea‐island cotton roots infected by wilt pathogen Verticillium dahliae[J]. Proteomics, 2011, 11(22): 4296-4309.
- Li Y, Feng H, Jin L, et al. A novel vector-based RNAi method using mouse U6 promoter-driven shRNA expression in the filamentous fungus Blakesleatrispora[J]. Biotechnology Letters, 2021, 43(9): 1821-1830.
- Shi J T, Li J. A method suitable for total protein extraction from wood forming tissue of Pinus koraiensis[C]//Advanced Materials Research. Trans Tech Publications Ltd, 2011, 179: 1199-1202.
- Yu X, Jones H D, Ma Y, et al. (E)-β-Farnesene synthase genes affect aphid (Myzuspersicae) infestation in tobacco (Nicotiana tabacum)[J]. Functional & integrative genomics, 2012, 12: 207-213.
- Wang F, Tong W, Zhu H, et al. A novel Cys 2/His 2 zinc finger protein gene from sweetpotato, IbZFP1, is involved in salt and drought tolerance in transgenic Arabidopsis[J]. Planta, 2016, 243: 783-797.
- Wang F, Zhu H, Kong W, et al. The Antirrhinum AmDEL gene enhances flavonoids accumulation and salt and drought tolerance in transgenic Arabidopsis[J]. Planta, 2016, 244: 59-73.
- Gao S, Yu B, Yuan L, et al. Production of transgenic sweetpotato plants resistant to stem nematodes using oryzacystatin-I gene[J]. Scientia Horticulturae, 2011, 128(4): 408-414.
- He T, Song B, Liu J, et al. A new isoform of thioredoxin h group in potato, SbTRXh1, regulates cold-induced sweetening of potato tubers by adjusting sucrose content[J]. Plant cell reports, 2012, 31: 1463-1471.
- Li L, Zhang X, Cui L, et al. Ursolic acid promotes the neuroprotection by activating Nrf2 pathway after cerebral ischemia in mice[J]. Brain research, 2013, 1497: 32-39.
- Ding Y, Chen M C, Wang M, et al. Neuroprotection by acetyl-11-keto-β-boswellic acid, in ischemic brain injury involves the Nrf2/HO-1 defense pathway[J]. Scientific reports, 2014, 4(1): 1-9.
- Li Y, Feng H, Jin L, et al. A novel vector-based RNAi method using mouse U6 promoter-driven shRNA expression in the filamentous fungus Blakesleatrispora[J]. Biotechnology Letters, 2021, 43(9): 1821-1830.
- Hu Y, Cai Y, Wang H, et al. Systematic Study of the Sensory Quality, Metabolomics, and Microbial Community of Fresh-Cut Watermelon Provides New Clues for Its Quality Control and Preservation[J]. Foods, 2022, 11(21): 3423.