SA-01-SK Minute™ 皮肤组织总蛋白提取试剂盒价格

英文名：Minute™ Total Protein Extraction Kit for Skin Tissue

保存条件：室温

规格：50 Preps

Minute^TM 皮肤组织总蛋白提取试剂盒

目录号：SA-01-SK

描述

皮肤组织由表皮、真皮和皮下脂肪组成。由于皮肤组织独特的结构，所以研磨匀浆非常困难，在提取总蛋白时也十分难裂解。使用传统的溶液蛋白质提取方法如RIPA，提取皮肤总蛋白效率，产率都极低。提取的蛋白质图谱也是不完整的。本试剂盒使用离心管柱技术及优化的裂解液，采取物理研磨和化学裂解相结合的方法，为人和动物皮肤组织总蛋白的提取提供了一种高效的方法。本试剂盒可简单快速的提取高质量皮肤总蛋白，无蛋白丢失，可以根据特定的下游应用选择变性细胞裂解液或天然细胞裂解液。整个提取过程不到10分钟即可完成，蛋白质得率在1-5mg/ml。试剂盒所提供试剂及耗材可以满足客户50个样品实验。

应用

SDS-PAGE, WB，IP，ELISA，酶活性测定和蛋白质组学分析。缓冲剂与IMAC树脂兼容，可用于His标签蛋白纯化。在质谱分析之前需将提取的蛋白质样品中的盐和表面活性剂除去。

参考文献：

Zhang, C., Li, Y., Qin, J., Yu, C., Ma, G., Chen, H., & Xu, X. (2021). TMT-Based Quantitative Proteomic Analysis Reveals the Effect of Bone Marrow Derived Mesenchymal Stem Cell on Hair Follicle Regeneration. Frontiers in Pharmacology, 12.
Wang, T., Zhu, G., Qin, L., Wang, Q., She, C., Xu, D., ... & Li, S. T. (2021). Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation. JID Innovations, 1(3), 100038.
Yin, S., Song, R., Ma, J., Liu, C., Wu, Z., Cao, G., ... & Wang, Y. (2022). Receptor activity‐modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis. Cell Communication and Signaling, 20(1), 1-18.

4.Wang, Y., Huang, J., & Jin, H. (2022). Reduction of Methyltransferase-like 3-Mediated RNA N6-Methyladenosine Exacerbates the Development of Psoriasis Vulgaris in Imiquimod-Induced Psoriasis-like Mouse Model. International Journal of Molecular Sciences, 23(20), 12672.

5.Zhu, W., Dong, Y., Xu, P., Pan, Q., Jia, K., Jin, P., ... & Che, B. (2022). A composite hydrogel containing resveratrol-laden nanoparticles and platelet-derived extracellular vesicles promotes wound healing in diabetic mice. Acta Biomaterialia.
6.Yuan, X., Tang, B., Chen, Y., Zhou, L., Deng, J., Han, L., ... & Wang, Y. (2023). Celastrol inhibits store operated calcium entry and suppresses psoriasis. Frontiers in Pharmacology, 14.

7.Zhou, H., Gan, Y., Li, Y., Chen, X., Guo, Y., & Wang, R. (2023). Degradation of Rat Sarcoma Proteins Targeting the Post-Translational Prenyl Modifications via Cascade Azidation/Fluorination and Click Reaction. Journal of Medicinal Chemistry.

8.Wang, J. Study on the Regulatory Mechanism of Jia Wei Guo Min Decoction On Degranulation of Mast Cell in Ad Mice Based on His/Par-2 Pathway.

9.Huang, J. L., Xu, Y. H., Yang, X. W., Wang, J., Zhu, Y., & Wu, X. B. (2023). Jiawei guomin decoction regulates the degranulation of mast cells in atopic dermatitis mice via the HIS/PAR-2 pathway. Journal of Ethnopharmacology, 117485.

该产品被引用文献

1.Zhang, C., Li, Y., Qin, J., Yu, C., Ma, G., Chen, H., & Xu, X. (2021). TMT-Based Quantitative Proteomic Analysis Reveals the Effect of Bone Marrow Derived Mesenchymal Stem Cell on Hair Follicle Regeneration. Frontiers in Pharmacology, 12.

2.Wang, T., Zhu, G., Qin, L., Wang, Q., She, C., Xu, D., ... & Li, S. T. (2021). Kininogen–Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation. JID Innovations, 1(3), 100038.

3.Yin, S., Song, R., Ma, J., Liu, C., Wu, Z., Cao, G., ... & Wang, Y. (2022). Receptor activity‐modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis. Cell Communication and Signaling, 20(1), 1-18.

4.Wang, Y., Huang, J., & Jin, H. (2022). Reduction of Methyltransferase-like 3-Mediated RNA N6-Methyladenosine Exacerbates the Development of Psoriasis Vulgaris in Imiquimod-Induced Psoriasis-like Mouse Model. International Journal of Molecular Sciences, 23(20), 12672.

5.Zhu, W., Dong, Y., Xu, P., Pan, Q., Jia, K., Jin, P., ... & Che, B. (2022). A composite hydrogel containing resveratrol-laden nanoparticles and platelet-derived extracellular vesicles promotes wound healing in diabetic mice. Acta Biomaterialia.

6.Yuan, X., Tang, B., Chen, Y., Zhou, L., Deng, J., Han, L., ... & Wang, Y. (2023). Celastrol inhibits store operated calcium entry and suppresses psoriasis. Frontiers in Pharmacology, 14.

7.Zhou, H., Gan, Y., Li, Y., Chen, X., Guo, Y., & Wang, R. (2023). Degradation of Rat Sarcoma Proteins Targeting the Post-Translational Prenyl Modifications via Cascade Azidation/Fluorination and Click Reaction. Journal of Medicinal Chemistry.

8.Wang, J. Study on the Regulatory Mechanism of Jia Wei Guo Min Decoction On Degranulation of Mast Cell in Ad Mice Based on His/Par-2 Pathway.

9.Huang, J. L., Xu, Y. H., Yang, X. W., Wang, J., Zhu, Y., & Wu, X. B. (2023). Jiawei guomin decoction regulates the degranulation of mast cells in atopic dermatitis mice via the HIS/PAR-2 pathway. Journal of Ethnopharmacology, 117485.