Anti-PRKCA antibody

供应商 ：江西江蓝纯生物试剂有限公司

库存 ：大量

克隆性 ：多克隆

保质期 ：1年

抗体英文名 ：Anti-PRKCA antibody

宿主 ：Rabbit

浓度 ：0.3 mg/ml

保存条件 ：-20°C

规格 ：25 μl/100 μl/200 μl

Anti-PRKCA antibody

Cat. No.     JC261348
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-PRKCA rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Synthetic peptide of human PRKCA
Reactivity                 Human, Mouse, Rat
Content                     0.3 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 PRKCA
Full name                 protein kinase C, alpha
Synonyms    AAG6; PKCA; PRKACA; PKC-alpha
Swissprot    P17252

Target Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human lung cancer
Recommended dilution: 25-100    
            
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml261348(PRKCA Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)    
Western blotting
Predicted band size:77 kDa
Positive control:Jurkat and NIH/3T3 cells 
Recommended dilution: 500-2000