CAS号 ://
供应商 :普利莱
保质期 :一年
保存条件 :2-8℃
英文名 :Phosphatase Inhibitors Mixture (All-in-One, 100)
库存 :大量现货
规格 :1ml/5ml
蛋白磷酸酶抑制剂混合物 (All-in-One, 100´) P1260 (抑制蛋白丝/苏氨酸磷酸酶、蛋白酪氨酸磷酸酶、碱性磷酸酶、酸性磷酸酶)
描述: 组织细胞在裂解过程中释放大量的内源性蛋白磷酸酶,能够以各种方式催化磷酸化蛋白的去磷酸化。本蛋白磷酸酶抑制剂混合物(All-in-One, 100x)包含6种独立的蛋白磷酸酶抑制剂,每一种抑制剂可特异性抑制某一种或几种蛋白磷酸酶活性,包括蛋白丝/苏氨酸磷酸酶(PP1、PP2A、PP2B、PP2C)、蛋白酪氨酸磷酸酶(PTPs)、碱性磷酸酶、酸性磷酸酶等。该混合物为100倍浓缩水溶液,可直接加入任何类型组织和细胞的裂解产物中,有效抑制磷酸化蛋白质的去磷酸化,从而维护蛋白质的磷酸化状态。适用于Western Blot和免疫共沉淀检测磷酸化蛋白质、测定蛋白激酶活性等实验。
参考文献:
1. Jain, M.K. (1982) Handbook of Enzyme Inhibitors, pp. 189-344. John Wiley and Sons, New York, NY.
2. Beynon, R. J., et al. (1989). Proteolytic Enzymes A Practical Approach. p.207
适用:Western Blot和免疫共沉淀检测磷酸化蛋白质、蛋白激酶活性测定等实验。
组成: 混合物为100倍浓缩水溶液,包含6种独立的蛋白磷酸酶抑制剂;强烈抑制所有蛋白磷酸酶的活性,包括蛋白丝/苏氨酸磷酸酶(PP1、PP2A、PP2B、PP2C)、蛋白酪氨酸磷酸酶(PTPs)、碱性磷酸酶、酸性磷酸酶等。
储存: 4 °C 12个月有效。储存期间溶液如果出现灰色,不影响使用。
使用方法:
1. 取出蛋白磷酸酶抑制剂混合物(All-in-One, 100x),振荡重悬。
2. 按照1:99比例,加入组织或细胞裂解液中,混合均匀 。
以下列举部分使用普利莱蛋白磷酸酶抑制剂发表的SCI文章供参考:
- Zhu L, Wang Z, Lin Y, et al. Sphingosine kinase 1 enhances the invasion and migration of non-small cell lung cancer cells via the AKT pathway[J]. Oncology reports, 2015, 33(3): 1257-1263.
- Zhou Y, Hu Z, Li N, et al. Interleukin-32 stimulates osteosarcoma cell invasion and motility via AKT pathway-mediated MMP-13 expression[J]. International journal of molecular medicine, 2015, 35(6): 1729-1733.
- Pu Y, Wang L, Wu H, et al. High MMP-21 expression in metastatic lymph nodes predicts unfavorable overall survival for oral squamous cell carcinoma patients with lymphatic metastasis[J]. Oncology reports, 2014, 31(6): 2644-2650.
- Cheng Z, Li-Sha G, Jing-Lin Z, et al. Protective Role of the Cholinergic Anti-Inflammatory Pathway in a Mouse Model of Viral Myocarditis[J]. PloS one, 2014, 9(11): e112719.
- Yang J D, Wang J C, Feng X M, et al. The isolation and cultivation of bone marrow stem cells and evaluation of differences for neural-like cells differentiation under the induction with neurotrophic factors[J]. Cytotechnology, 2014, 66(6): 1007-1019.
- Fang X, Gao G, Xue H, et al. Exposure of perfluorononanoic acid suppresses the hepatic insulin signal pathway and increases serum glucose in rats[J]. Toxicology, 2012, 294(2): 109-115.
该产品被引用文献
- Chen J, Yang L, Geng L, et al. Inhibition of acyl-CoA synthetase long-chain family member 4 facilitates neurological recovery after stroke by regulation ferroptosis[J]. Frontiers in Cellular Neuroscience, 2021, 15: 632354.
- Chang S, Hu L, Xu Y, et al. Inorganic nitrate alleviates total body irradiation-induced systemic damage by decreasing reactive oxygen species levels[J]. International Journal of Radiation Oncology* Biology* Physics, 2019, 103(4): 945-957.
- Dong L, Li R, Li D, et al. FGF10 enhances peripheral nerve regeneration via the preactivation of the PI3K/Akt signaling-mediated antioxidant response[J]. Frontiers in Pharmacology, 2019, 10: 1224.
- Zhang Z, Liu H, Zhao Z, et al. GJ-4 alleviates Aβ25-35-induced memory dysfunction in mice through protecting the neurovascular unit[J]. Biomedicine & Pharmacotherapy, 2020, 127: 110131.
- Chen J, Zhang X, Liu X, et al. Ginsenoside Rg1 promotes cerebral angiogenesis via the PI3K/Akt/mTOR signaling pathway in ischemic mice[J]. European journal of pharmacology, 2019, 856: 172418.
- Ding C, Li Y, Wang S, et al. ROBO2 hampers malignant biological behavior and predicts a better prognosis in pancreatic adenocarcinoma[J]. Scandinavian Journal of Gastroenterology, 2021, 56(8): 955-964.
- Shao M, Wang M, Ma L, et al. β-elemene blocks lipid-induced inflammatory pathways via PPARβ activation in heart failure[J]. European Journal of Pharmacology, 2021, 910: 174450.
- Zhang J, Lin W, Tang M, et al. Inhibition of JNK ameliorates depressive-like behaviors and reduces the activation of pro-inflammatory cytokines and the phosphorylation of glucocorticoid receptors at serine 246 induced by neuroinflammation[J]. Psychoneuroendocrinology, 2020, 113: 104580.
- Qiu L Z, Zhou W, Yue L X, et al. Repeated Aconitine Treatment Induced the Remodeling of Mitochondrial Function via AMPK–OPA1–ATP5A1 Pathway[J]. Frontiers in Pharmacology, 2021, 12: 646121.
- Liu X, Du Y, Liu J, et al. TUK9Ferrostatin-1 alleviates cerebral ischemia/reperfusion injury through activation of the AKT/GSK3β signaling pathway[J]. Brain Research Bulletin, 2022.
- Yang L, Cheng J, Shi G, et al. Liraglutide ameliorates cerebral ischemia in mice via antipyroptotic pathways[J]. Neurochemical Research, 2022, 47(7): 1904-1916.
- Wu C, Zheng W, Jia X, et al. Adolescent chronic unpredictable stress causes a bias in goal‐directed behavior and distinctively changes the expression of NMDA and dopamine receptors in the dorsomedial and dorsolateral striatum in male rats[J]. Developmental Psychobiology, 2022, 64(2): e22235.
- Jiang M, Wang W, Zhang J, et al. Protective effects and possible mechanisms of actions of BushenCuyun recipe on diminished ovarian reserve induced by cyclophosphamide in rats[J]. Frontiers in Pharmacology, 2020, 11: 546.
- Li D, Wang C, Chi C, et al. Bone marrow mesenchymal stem cells inhibit lipopolysaccharide-induced inflammatory reactions in macrophages and endothelial cells[J]. Mediators of Inflammation, 2016, 2016.
- Shen Y, Li M, Xiong Y, et al. Proteomics analysis identified ASNS as a novel biomarker for predicting recurrence of skull base chordoma[J]. Frontiers in oncology, 2021, 11: 698497.
- Du J, Fang J, Wen C, et al. The effect of electroacupuncture on PKMzeta in the ACC in regulating anxiety-like behaviors in rats experiencing chronic inflammatory pain[J]. Neural plasticity, 2017, 2017.
- Zhang Y, Shen Y, Li M, et al. Loss of INI1 inhibits the expression of SIDT1 and promotes tumor progression in skull base chordoma by regulating EZH2-mediated H3K27me3[J]. 2022.
- Wang B, Hu C, Yang X, et al. Inhibition of GSK-3β activation protects SD rat retina against N-methyl-N-nitrosourea-induced degeneration by modulating the Wnt/β-catenin signaling pathway[J]. Journal of Molecular Neuroscience, 2017, 63: 233-242.
- Liu Q, Shen Y, Xiong Y, et al. Comparative Efficacy of AZD9496 and Fulvestrant on the Growth of Pituitary Adenoma via Blocking JAK2/STAT5B Pathway[J]. Journal of Cancer, 2023, 14(1): 61-71.
- Liu W, Li J, Cai Y, et al. Hepatic IGF-1R overexpression combined with the activation of GSK-3β and FOXO3a in the development of liver cirrhosis[J]. Life sciences, 2016, 147: 97-102.