Phospho-Akt (Ser473) (587F11) (Biotinylated)

保存条件 ：低温

保质期 ：一年

英文名 ：Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated)

库存 ：大量

供应商 ：科敏商城

CAS号 ：IP ,

规格 ：200 ul (200 immunoprecipitations)

品牌:CST，产品名称：Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated) #5102
英文名称：Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated)
Product name：Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated) #5102
Applications：IP ,
Purification： Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Serine 473 of mouse Akt.
Background： Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).

Entrez-Gene Id

207, 208, 10000

Swiss-Prot Acc.

P31749, P31751, Q9Y243

Specificity / Sensitivity:

Phospho-Akt (Ser473) (587F11) Mouse mAb detects endogenous levels of Akt only when phosphorylated at Ser473. This antibody does not detect Akt phosphorylated at other sites or related kinases such as PKC and p70 S6 kinase. This is a biotinylated version of the #4051 antibody and is expected to continue to recognize other species such rat and hamster.

Species Reactivity:

Human, Mouse, Rat, Hamster

Species predicted to react based on 100% sequence homology:

Monkey

Application	Dilutions
Immunoprecipitation	1:50

Storage:

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.