Phospho-eNOS (Ser1177) ELISA

保存条件 ：低温

保质期 ：一年

英文名 ：PathScan® Phospho-eNOS (Ser1177) Sandwich ELISA Kit

库存 ：大量

供应商 ：科敏商城

规格 ：1 Kit (96 assays)

品牌:CST，产品名称：PathScan Phospho-eNOS (Ser1177) Sandwich ELISA Kit #7980
英文名称：PathScan® Phospho-eNOS (Ser1177) Sandwich ELISA Kit
Product name：PathScan^® Phospho-eNOS (Ser1177) Sandwich ELISA Kit #7980
Isotype：Rabbit IgG
Sensitivity：Endogenous
Background： Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H₂O₂ activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).

Entrez-Gene Id

4846

Swiss-Prot Acc.

P29474

Specificity / Sensitivity:

PathScan^® Phospho-eNOS (Ser1177) Sandwich ELISA Kit #7980 detects endogenous levels of eNOS protein only when phosphorylated at Ser1177 (see Figure 1). The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Bovine

Product Description:

The PathScan^® Phospho-eNOS (Ser1177) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of eNOS when phosphorylated at Ser1177. A phospho-eNOS (Ser1177) rabbit monoclonal antibody has been coated onto the microwells. After incubation with cell lysates, phospho-eNOS protein is captured by the coated antibody. Following extensive washing, an eNOS mouse monoclonal detection antibody is added to detect captured eNOS protein phosphorylated at Ser1177. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate,TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of eNOS phosphorylated at Ser1177.

Antibodies in kit are custom formulations specific to kit.