p44/42 MAPK (Erk1/2) (L34F12)

保存条件 ：低温

保质期 ：一年

英文名 ：p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb

库存 ：大量

供应商 ：科敏商城

CAS号 ：WB , IHC , IF , F ,

规格 ：100 ul (20 western blots)

品牌:CST，产品名称：p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb #4696
英文名称：p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb
Product name：p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb #4696
Isotype：Mouse IgG1
Sensitivity：Endogenous
MW(kDa)：42, 44
Applications：WB , IHC , IF , F ,
Purification： Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of p42 MAP Kinase.
Background： Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.

Entrez-Gene Id

5595, 5594

Swiss-Prot Acc.

P27361, P28482

Specificity / Sensitivity:

p44/42 MAP Kinase (L34F12) Mouse mAb detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some systems this antibody may recognize p42/Erk2 more readily than p44/Erk1. The antibody does not cross-react with JNK/SAPK or p38 MAP kinase.

Species Reactivity:

Human, Mouse, Rat, Monkey, Mink, Zebrafish, Bovine, Pig

Application	Dilutions
Western Blotting	1:2000
Immunohistochemistry (Paraffin)	1:500
Immunofluorescence (Immunocytochemistry)	1:100
Flow Cytometry	1:200

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.