Phospho-NF-κB p65 (Ser536) (93H1) (Biotinylated)

保存条件 ：低温

保质期 ：一年

英文名 ：Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)

库存 ：大量

供应商 ：科敏商城

CAS号 ：WB ,

规格 ：100 ul (10 western blots)

品牌:CST，产品名称：Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated) #4025
英文名称：Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)
Product name：Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated) #4025
Applications：WB ,
Purification： Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-κB p65 protein.
Background： Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).

Entrez-Gene Id

5970

Swiss-Prot Acc.

Q04206

Specificity / Sensitivity:

Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated) detects NF-κB p65 protein only when phosphorylated at Ser536. It does not cross-react with the p50 subunit or other related proteins.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey, Pig

Species predicted to react based on 100% sequence homology:

Dog

Application	Dilutions
Western Blotting	1:1000

Storage:

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Product Description:

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 reacts with human, mouse, rat, monkey, hamster, and pig phospho-NF-κB. CST expects that Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated) will also recognize phospho-NF-κB in these species.