Phospho-c-Raf (Ser296) Antibody

保存条件 ：低温

保质期 ：一年

英文名 ：Phospho-c-Raf (Ser296) Antibody

库存 ：大量

供应商 ：科敏商城

CAS号 ：WB ,

规格 ：100 ul (10 western blots)

品牌:CST，产品名称：Phospho-c-Raf (Ser296) Antibody #9423
英文名称：Phospho-c-Raf (Ser296) Antibody
Product name：Phospho-c-Raf (Ser296) Antibody #9423
Isotype：Rabbit IgG
Sensitivity：Endogenous
MW(kDa)：86
Applications：WB ,
Purification： Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser296 of human c-Raf. Antibodies are purified by protein A and peptide affinity chromatography.
Background： A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8,9). Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301, and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events (11).

Entrez-Gene Id

5894

Swiss-Prot Acc.

P04049

Specificity / Sensitivity:

Phospho-c-Raf (Ser296) Antibody detects endogenous levels of c-Raf protein only when phosphorylated at Ser296.

Species Reactivity:

Human, Mouse, Rat

Application	Dilutions
Western Blotting	1:1000

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.