GGA3 (D66F1)

保存条件 ：低温

保质期 ：一年

英文名 ：GGA3 (D66F1) Rabbit mAb

库存 ：大量

供应商 ：科敏商城

CAS号 ：WB , IP ,

规格 ：100 ul (10 western blots)

品牌:CST，产品名称：GGA3 (D66F1) Rabbit mAb #8027
英文名称：GGA3 (D66F1) Rabbit mAb
Product name：GGA3 (D66F1) Rabbit mAb #8027
Isotype：Rabbit IgG
Sensitivity：Endogenous
MW(kDa)：90
Applications：WB , IP ,
Purification： Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu523 of human GGA3 protein.
Background： GGA3 is a member of the GGA family of proteins which also includes GGA1 and GGA2. These proteins consist of four distinct segments: a VHS domain that binds the di-leucine sorting signal DXXLL; a GAT domain that binds Arf-GTP; a hinge region that recruits clathrin; and a GAE domain that has sequence similarity to γ-adaptin and recruits a number of proteins. Arf1-GTPase recruits GGA3 to the trans-Golgi network. GGAs sort acid hydrolases to the lysosome and are involved in transporting proteins containing the DXXLL signal from the Golgi complex to the endosome (1). During apoptosis or cerebral ischemia, GGA3 is cleaved by caspase-3 at Asp313, reducing GGA3 levels and lysosomal degradation of β-secretase (BACE). The resulting elevated amount and activity of BACE plays a role in amyloid-β (Aβ) production, consistent with BACE elevation and Aβ accumulation in Alzheimer’s Disease (2).

Entrez-Gene Id

23163

Swiss-Prot Acc.

Q9NZ52

Specificity / Sensitivity:

GGA3 (D66F1) Rabbit mAb recognizes endogenous levels of total GGA3 protein. This antibody may also recognize cross-reacting bands of unknown origin between 25 and 32 kDa.

Species Reactivity:

Human, Mouse, Rat

Application	Dilutions
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.