Cyclic AMP Select ELISA Kit

Technical Information

Synonyms

Adenosine 3',5'-cyclic mononucleotide
Adenosine 3',5'-cyclic monophosphate
cAMP EIA Kit

Assay Range

0.078-10 pmol/ml

Sensitivity

80% B/B₀: 0.1 pmol/ml

Mid-point

50% B/B₀: 0.5 pmol/ml

Cross Reactivity

(Non-Acetylated:)
(cAMP) 100%
(cGMP) 1.5%
(Adenosine) <0.01%
(AMP) <0.01%
(ATP) <0.01%
(Acetylated:)
(Acetylated cAMP) 100%
(Acetylated cGMP) 0.69%
(Acetylated adenosine) <0.01%
(Acetylated AMP) <0.01%

Origin

Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit

Cayman’s cAMP assay is a very sensitive competitive ELISA that permits cAMP measurements within the standard curve range of 0.078-10 pmol/ml, typically with a sensitivity (80% B/B₀) of 0.1 pmol/ml once samples and standards have been acetylated prior to analysis. This assay has been validated with culture media, urine, tissue, and plasma samples. If you prefer to assay your samples and still achieve excellent sensitivity without the need to perform a pre-assay acetylation step we recommend using Cayman’s cAMP Select ELISA Kit.