磷酸化原癌基因Yes相关蛋白1抗体品牌

磷酸化原癌基因Yes相关蛋白1抗体品牌

价格: 询价

品牌:上海一研

货号:EY-3349K

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抗体名 :磷酸化原癌基因Yes相关蛋白1抗体品牌

抗体英文名 :Anti-Phospho-YAP1(Tyr407)

靶点 :详见说明书

浓度 :1mg/1ml 

应用范围 :产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

宿主 :详见说明书

供应商 :上海一研

库存 :29

级别 :详见说明书

目录编号 :详见说明书

抗原来源 :Rabbit

保质期 :详见说明书

适应物种 :详见说明书

标记物 :详见说明书

克隆性 :多克隆

保存条件 :Store at -20 °C

形态 :详见说明书

亚型 : IgG 

免疫原 :KLH conjugated Synthesised phosphopeptide derived from human YAP around the phosphorylation site of Tyr407

规格 :0.1ml/100μg

0.1ml/100μg
磷酸化原癌基因Yes相关蛋白1抗体品牌CL-0300NCI-H661(人大细胞肺癌细胞)5×106cells/瓶×2
IL1R1 Others Mouse 小鼠 IL1R1 / CD121a 人细胞裂解液 (阳性对照)
小鼠胰腺星状细胞完全培养基 100mL
A9小鼠皮下结缔组织细胞 A9 mice subcutaneous connective tissue cells DMEM培养基+10%FBS
IL1B Protein Rat 重组大鼠 IL-1 beta / IL1B 蛋白 (pro form, His 标签)
NEC(人食管癌细胞) 5×106cells/瓶×2 NIH/3T3(胚胎成纤维细胞)
英文名称  Anti-Phospho-YAP1(Tyr407) 
中文名称 磷酸化原癌基因Yes相关蛋白1抗体品牌
   65 kDa Yes associated protein; YAp 1; YAp-1; YAP 65; YAP; YAP2; YAP65; Yes associated protein 1 65kDa; Yes associated protein 1; Yes associated protein 2; YKI.

1mg/1ml
0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子
蛋白分子量 predicted molecular weight
55kDa  
Lyophilized or Liquid
KLH conjugated Synthesised phosphopeptide derived from human YAP around the phosphorylation site of Tyr407
IgG
纯化方法 affinity purified by Protein A
0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 YAP1 (also known as Yes associated protein 1) was originally identified as a transcription factor that binds to the SH3 domain of the YES kinase (a Src protein kinase). More recently it has been identified as a candidate oncogene that promotes tumorigenesis in many different types of cancer.
Function
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).  

公司专业供应的抗体,磷酸化原癌基因Yes相关蛋白1抗体品牌是用于化学反应、分析化验、研究实验、教学实验、化学配方使用的纯净化学品,产品品质卓越,价格实惠,多种规格供应,售后完善。
磷酸化原癌基因Yes相关蛋白1抗体品牌几种抗体概述:
1.抗角蛋白抗体(AKA),又称为抗丝集蛋白抗体或抗角质层抗体。AKA是类风湿关节炎早期诊断和判断预后的指标之一,其对RA患者的诊断的特异性为94% ,而其敏感性为47% ,并是一种鉴别RA和与多发性关节炎相关的丙型肝炎患者的有效检验标记物。
2.抗核周因子( APF), 是定位于口腔黏附膜细胞胞质内的颗粒性蛋白复合物。可在RA患者的血清和关节液中测出,与性别、年龄无关。APF显示了较强的特异性(73%99%)和敏感性。用间接免疫荧光法检测,方法精密度较差。
3.抗环瓜氨酸肽抗体(CCP),具有相对好的敏感度和特异性。其实,抗核周因子和抗角蛋白抗体,与CCP在化学结构上具有相关性,它们的表位都含有瓜氨酸,称为瓜氨酸相关自身免疫系统。
实验原理 :
1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
2)活补体:IgMIgG1IgG2IgG3可通过经典途径激活补体,凝聚的IgAIgG4IgE可通过替代途径激活补体。
3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
4)可通过胎盘及粘膜:免疫球蛋白GIgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白AIgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,6070℃即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。

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磷酸化原癌基因Yes相关蛋白1抗体品牌大鼠基质金属蛋白酶抑制剂-3(rat TIMP-3)   作用:   ELISA   规格:   48T/96T   进口分装
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