文献支持1 | PEB(4x)| 蛋白质提取缓冲液|1 | PEB (4x) | protein extraction buffer
价格: ¥11
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保存条件 :Stable at RT for at least 1 month
保质期 :1
库存 :100
供应商 :艾美捷科技
应用范围 :protein extraction
抗体英文名 :1 | PEB (4x) | protein extraction buffer
抗体名 :1 | PEB(4x)| 蛋白质提取缓冲液
规格 :5x2ml
关键词:1 | PEB (4x) | protein extraction buffer,1 | PEB(4x)| 蛋白质提取缓冲液,1 | PEB(4x)| 蛋白质提取缓冲液
产品名称:1 | PEB(4x)| 蛋白质提取缓冲液-1 | PEB (4x) | protein extraction buffer
产品货号:AS08-300
产品规格:5x2ml
应用类型:protein extraction
保存建议:1 | PEB(4x)| 蛋白质提取缓冲液-1 | PEB (4x) | protein extraction bufferStable at RT for at least 1 month; short-term storage (6 monthss) at 4°C and long term storage (1 year or more) at -20°C.
背景资料:PEB is an extraction buffer for disruption and solubilisation of total protein from plant tissue and algal cells. The use of the anionic detergent LDS together with the recommended procedure (combination of sonication and freeze/thaw cycles) has been shown to increase the number of solubilised and non-degraded proteins when compared to other methods of cell disruption (see reference). The estimated hands-on time for the recommended procedure is 20-30 minutes for 1-2 samples. Expected yields will be 1.5-6 µg/µl total protein (recovered from standard procedure) depending on the starting material, e.g. its biological stage, homogenization method used (bead beater vs. sonication).
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