常规siRNA价格_品牌:锐博生物-丁香通官网

保存条件：-20℃~-80℃

规格：5nmol

常规siRNA为常规化学合成的、未经任何化学修饰的双链RNA，特异性靶点设计，覆盖人、小鼠、大鼠全基因组的所有基因（包括mRNA、lncRNA、circRNA等），其他物种亦可定制，适合进行各种细胞RNAi实验。

常规siRNA的结构多为19nt RNA + dTdT (3'悬垂) 的RNA双链分子，其中Sense链（正义链，RNA）序列与靶序列相同，Antisense链（反义链，RNA）序列与靶序列互补，在每条链的3'端还有一个dTdT悬垂。

常规化学合成siRNA 结构示例图
siRNA for Homo sapiens gene bcl-2-like protein 11（BCL2L11）

产品优势

1、采用siCatch™ siRNA design智能设计；
2、通过系统性优化，保证靶点特异性；
3、均经过严格的质谱检测，保证siRNA质量和纯度；
4、只需溶解，即可直接用于实验。

产品特性

■ 形式：双链，已退火
■ 剂型：冻干粉
■ 质控：LC-MS 质控管理
■ 使用：只需溶解，即可直接用于实验

化学合成siRNA 单链 LC-MS 质控结果

5nmol siRNA 的筛选次数参考表

孔板规格	6	12	24	96
工作浓度	50nM	50nM	50nM	50nM
筛选次数	50次	100次	200次	1000次

客户应用案例

案例1：基因特异性siRNA

将siASGR1和siCtrl转染到人肝癌细胞系Huh7，结果发现转染siASGR1显著降低了ASGR1的相对mRNA水平。并且导致经典LXR靶基因（包括
ABCG5，ABCG8和ABCA1）的表达升高。

Wang JQ, et al. Nature, 2022.

案例2：lncRNA特异性siRNA

将两个独立的靶向LINC00680的siRNAs转染KYSE510细胞，结果显示转染si LINC00680显著降低了LINC00680的相对表达，且LINC00680的敲低对细胞增殖的影响最为显著。

Xue S, et al. Molecular cancer, 2022.

案例3：circRNA特异性siRNA

将特异性靶向circEXOC6B的siRNAs转染CRC细胞（SW620、HCT116），结果发现转染si1-circEXOC6B和si2-circEXOC6B显著降低了circEXOC6B的表达水平，并且敲低circEXOC6B显著促进了细胞的增殖。

Li X, et al. Molecular cancer, 2022.

订购信息

锐博生物官网（www.ribobio.com）支持24h在线订购产品或委托科研服务，如需订购常规siRNA产品，只需搜索基因名、产品名、产品编号等，选择您所需要的siRNA 产品加入购物车即可完成下单购买，省时又方便！锐博在线平台专为广大科研工作者提供及时、贴心、专业、优质的核酸产品或服务，让每一个科研人员省心、放心，为每一个科学研究保驾护航！

产品编号	产品名称	规格	目录价
Varies	mRNA/lncRNA/circRNA siRNA, Standard, 5nmol	5nmol	¥589.00
Varies	mRNA/lncRNA/circRNA siRNA, Standard, 5nmol	5nmol*2	¥950.00
Varies	mRNA/lncRNA/circRNA siRNA, Standard, 5nmol	5nmol*4	¥1280.00

注：
1) 上述报价以19nt+3'overhangs（2nt）siRNA 作为标准，超过部分碱基按RNA oligo 合成单价的相应合成规格进行收费；
2) 如需化学修饰部分按化学修饰产品单价的相应合成规格进行收费；
3) Standard 纯化通常用于细胞实验，in vivo 纯化普遍适用于细胞或动物实验；
4) 如需咨询更大规格产品，可联系当地锐博销售人员。

代表性客户文章

[1] GE, Li-Ping, et al. ZNF689 deficiency promotes intratumor heterogeneity and immunotherapy resistance in triple-negative breast cancer. Cell Research, 2024, 1-18.
[2] XU, Zhi-Sheng, et al. LDLR is an entry receptor for Crimean-Congo hemo rrhagic fever v ir us. Cell Research, 2024, 1-11.
[3] LIN, Xiaolei, et al. Augmentation of scleral glycolysis promotes myopia through histone lactylation. Cell Metabolism, 2024.
[4] WANG, Ailian, et al. Innate immune sensing of lysosomal dysfunction drives multiple lysosomal storage disorders. Nature Cell Biology, 2024, 1-16.
[5] GAO, Jinchao, et al. Intracerebral fate of organic and inorganic nanoparticles is dependent on microglial extracellular vesicle function. Nature Nanotechnology, 2023, 1-11.
[6] ZENG, Qin, et al. Spleen fibroblastic reticular cell-derived acetylcholine promotes lipid metabolism to drive autoreactive B cell responses. Cell Metabolism, 2023, 35.5: 837-854. e8.
[7] LIU, Xuanyu, et al. Lineage-specific regulatory changes in hypertrophic cardiomyopathy unraveled by single-nucleus RNA-seq and spatial transcriptomics. Cell Discovery, 2023, 9.1: 6.
[8] Wang J Q, Li L L, Hu A, et al. Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion[J]. Nature, 2022, 608(7922): 413-420.
[9] Qi Y, Ding L, Zhang S, et al. A plant immune protein enables broad antitumor response by rescuing microRNA deficiency[J]. Cell, 2022, 185(11): 1888-1904. e24.
[10] Ma S, Wang S, Ye Y, et al. Heterochronic parabiosis induces stem cell revitalization and systemic rejuvenation across aged tissues[J]. Cell Stem Cell, 2022.
[11] Wu B, Li Y, Nie N, et al. SFRP4+ stromal cell subpopulation with IGF1 signaling in human endometrial regeneration [J]. Cell discovery, 2022, 8(1): 1-16.
[12] Ren Y, Wang A, Wu D, et al. Dual inhibition of innate immunity and apoptosis by human cytomegalovirus protein UL37x1 enables eff icient virus r eplication[J]. Nature Microbiology, 2022: 1-13.
[13] Liu F, Tian T, Zhang Z, et al. Long non-coding RNA SNHG6 couples cholesterol sensing with mTORC1 activation in hepatocellular carcinoma[J]. Nature Metabolism, 2022, 4(8): 1022-1040.
[14] Zhang D, Liu Y, Zhu Y, et al. A non-canonical cGAS–STING–PERK pathway facilitates the translational program critical for senescence and or gan fibrosis[J]. Nature Cell Biology, 2022, 24(5): 766-782.

该产品被引用文献

[1] GE, Li-Ping, et al. ZNF689 deficiency promotes intratumor heterogeneity and immunotherapy resistance in triple-negative breast cancer. Cell Research, 2024, 1-18.
[2] XU, Zhi-Sheng, et al. LDLR is an entry receptor for Crimean-Congo hemo rrhagic fever v ir us. Cell Research, 2024, 1-11.
[3] LIN, Xiaolei, et al. Augmentation of scleral glycolysis promotes myopia through histone lactylation. Cell Metabolism, 2024.
[4] WANG, Ailian, et al. Innate immune sensing of lysosomal dysfunction drives multiple lysosomal storage disorders. Nature Cell Biology, 2024, 1-16.
[5] GAO, Jinchao, et al. Intracerebral fate of organic and inorganic nanoparticles is dependent on microglial extracellular vesicle function. Nature Nanotechnology, 2023, 1-11.
[6] ZENG, Qin, et al. Spleen fibroblastic reticular cell-derived acetylcholine promotes lipid metabolism to drive autoreactive B cell responses. Cell Metabolism, 2023, 35.5: 837-854. e8.
[7] LIU, Xuanyu, et al. Lineage-specific regulatory changes in hypertrophic cardiomyopathy unraveled by single-nucleus RNA-seq and spatial transcriptomics. Cell Discovery, 2023, 9.1: 6.
[8] Wang J Q, Li L L, Hu A, et al. Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion[J]. Nature, 2022, 608(7922): 413-420.
[9] Qi Y, Ding L, Zhang S, et al. A plant immune protein enables broad antitumor response by rescuing microRNA deficiency[J]. Cell, 2022, 185(11): 1888-1904. e24.
[10] Ma S, Wang S, Ye Y, et al. Heterochronic parabiosis induces stem cell revitalization and systemic rejuvenation across aged tissues[J]. Cell Stem Cell, 2022.
[11] Wu B, Li Y, Nie N, et al. SFRP4+ stromal cell subpopulation with IGF1 signaling in human endometrial regeneration [J]. Cell discovery, 2022, 8(1): 1-16.
[12] Ren Y, Wang A, Wu D, et al. Dual inhibition of innate immunity and apoptosis by human cytomegalovirus protein UL37x1 enables eff icient virus r eplication[J]. Nature Microbiology, 2022: 1-13.
[13] Liu F, Tian T, Zhang Z, et al. Long non-coding RNA SNHG6 couples cholesterol sensing with mTORC1 activation in hepatocellular carcinoma[J]. Nature Metabolism, 2022, 4(8): 1022-1040.
[14] Zhang D, Liu Y, Zhu Y, et al. A non-canonical cGAS–STING–PERK pathway facilitates the translational program critical for senescence and or gan fibrosis[J]. Nature Cell Biology, 2022, 24(5): 766-782.