Anti-NUDT2 antibody

供应商 ：江西江蓝纯生物试剂有限公司

库存 ：大量

克隆性 ：多克隆

保质期 ：1年

抗体英文名 ：Anti-NUDT2 antibody

宿主 ：Rabbit

浓度 ：0.6 mg/ml

保存条件 ：-20°C

规格 ：25 μl/100 μl/200 μl

Anti-NUDT2 antibody

Cat. No.     JLC223794        
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-NUDT2 rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Full length fusion protein
Reactivity                 Human, Mouse, Rat
Content                     0.6 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 NUDT2
Full name                 nudix (nucleoside diphosphate linked moiety X)-type motif 2
Synonyms    APAH1
Swissprot    P50583

Target Background
This gene encodes a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. The gene product possesses a modification of the MutT sequence motif found in certain nucleotide pyrophosphatases. The enzyme asymmetrically hydrolyzes Ap4A to yield AMP and ATP and is responsible for maintaining the intracellular level of the dinucleotide Ap4A, the function of which has yet to be established. This gene may be a candidate tumor suppressor gene.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 20-100    Predicted cell location: Cytoplasm
Positive control: Human lung cancer
Recommended dilution: 20-100
            
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml223794(NUDT2 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml223794(NUDT2 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:17 kDa
Positive control:Mouse kidney , spleen and liver tissue,Jurkat cell,Hepg2 cell,293T cell,Human placenta tissue 
Recommended dilution: 200-1000

Gel: 12%SDS-PAGE
Lysate: 40 μg
Lane 1-7: Mouse kidney , spleen and liver tissue,Jurkat cell,Hepg2 cell,293T cell,Human placenta tissue
Primary antibody: ml223794(NUDT2 Antibody) at dilution 1/250
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 40 seconds
    
ELISA
Recommended dilution: 5000-10000