Anti-MAP3K7 antibody

供应商 ：江西江蓝纯生物试剂有限公司

库存 ：大量

克隆性 ：多克隆

保质期 ：1年

抗体英文名 ：Anti-MAP3K7 antibody

宿主 ：Rabbit

浓度 ：0.3 mg/ml

保存条件 ：-20°C

规格 ：25 μl/100 μl/200 μl

Anti-MAP3K7 antibody

Cat. No.     JLC223285        
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-MAP3K7 rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Fusion protein of human MAP3K7
Reactivity                 Human, Mouse, Rat
Content                     0.3 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 MAP3K7
Full name                 mitogen-activated protein kinase kinase kinase 7
Synonyms    TAK1; MEKK7; TGF1a
Swissprot    O43318

Target Background
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm and Cell membrane
Positive control: Human esophagus cancer
Recommended dilution: 10-50    Predicted cell location: Cytoplasm and Cell membrane
Positive control: Human lung cancer
Recommended dilution: 10-50
            
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml223285(MAP3K7 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml223285(MAP3K7 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:67 kDa
Positive control:HEPG2 cell lysate 
Recommended dilution: 500-2000

Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane: HEPG2 cell lysate
Primary antibody: ml223285(MAP3K7 Antibody) at dilution 1/250
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 1 minute
    
ELISA
Recommended dilution: 2000-5000