Anti-STX18 antibody

供应商 ：江西江蓝纯生物试剂有限公司

库存 ：大量

克隆性 ：多克隆

保质期 ：1年

抗体英文名 ：Anti-STX18 antibody

宿主 ：Rabbit

浓度 ：0.4 mg/ml

保存条件 ：-20°C

规格 ：25 μl/100 μl/200 μl

Anti-STX18 antibody

Cat. No.     JLC223273        
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-STX18 rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Fusion protein of human STX18
Reactivity                 Human, Mouse, Rat
Content                     0.4 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 STX18
Full name                 syntaxin 18
Synonyms    Ufe1
Swissprot    Q9P2W9

Target Background
Syntaxins, a family of proteins involved in the fusion of synaptic vesicles with the plasma membrane, display broad tissue distribution and contain carboxy-terminal hydrophobic domains that direct themselves to their respective intracellular compartments. Syntaxin 18 mainly localizes to the endoplasmic reticulum (ER), and functions in transport between the ER and Golgi. Syntaxin 18 also plays a role in ER-mediated phagocytosis, possibly by regulating the specific fusion of the ER and plasma or phagosomal membranes.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 25-100    Predicted cell location: Cytoplasm
Positive control: Human breast cancer
Recommended dilution: 25-100
            
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml223273(STX18 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml223273(STX18 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:39 kDa
Positive control:Raw264.7 and TM4 cells 
Recommended dilution: 200-1000

Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-2: Raw264.7, TM4 cells
Primary antibody: ml223273(STX18 Antibody) at dilution 1/350
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 20 seconds
    
ELISA
Recommended dilution: 1000-2000