产品详情
文献和实验
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保质期 ：一年
保存条件 ：4℃
规格 ：100ml
无蛋白快速封闭液(5×)
Protein Free Rapid Blocking Buffer(5×)
本产品常温运输；保存于4℃，保质期12个月。
货号规格
goodPS108 100 mL
产品特点
gd快速高效——5~10 min快速完成封闭；只结合封闭转印膜而不结合蛋白，因此不会屏蔽样品蛋白的抗体识别位点，与传统的脱脂奶粉和BSA封闭相比信噪比更高；
gd性能可靠——无蛋白配方，与抗体无交叉反应；
gd应用范围广——无磷酸化蛋白，无生物素，尤其适合磷酸化特异抗体及生物素检测系统；
gd节省抗体——使用本封闭液后，所需的最适抗体浓度低于常规使用浓度，可大大节省抗体使用量。
产品说明
good本品专为快速高效封闭Western Blot转印膜而设计，采用无蛋白配方，避免抗体与封闭剂的非特异结合，可有效降低背景。同时，本封闭剂无内源的磷酸化蛋白及生物素，对磷酸化特异抗体及生物素检测也不会产生干扰。本封闭剂不与蛋白产生非特异性结合，因而只封闭膜而不会屏蔽样品蛋白的抗体识别位点，大大提高了抗体检测灵敏度。实验表明，本封闭剂对许多抗体都有不同程度的信号增强作用。然而这种信号增强作用因抗体而异，对某一特定抗体，并不一定具有确定的信号增强作用。
good注意：没有任何一种封闭剂能适合所有抗原和抗体检测系统。如出现本试剂不适合的抗体，请替换使用其它种类的封闭剂如BSA或脱脂奶粉等。
封闭方法
good1. 完成转膜后，将转印膜放入到杂交孵育盒中，加入10~20 mL TBS/T或PBS/T；
good2. 往TBS/T或PBS/T中加入1/4体积的 无蛋白快速封闭液 ，置于摇床轻轻摇动，室温封闭约10 min(即配即用)；
good2. 注意：使用本品通常封闭5~15 min均可；经多种抗体的测试封闭10 min的效果显著优于常规的BSA封闭1 h。对于一些背景非常高的抗体，可以尝试将封闭时间延长为30~60 min。如有特殊需要，也可4℃封闭过夜。
good3. 封闭后的膜即可用于一抗孵育等后续实验。
注意事项
good1. 本产品也可以用于免疫组化 (IHC) 和免疫荧光 (IF) 实验的封闭操作；
good2. 为了您的安全和健康，请穿实验服并戴一次性手套操作；
good3. 本产品仅限科研使用。
相关论文
good1. Liu Z, Wang T, She Y, et al. N6-methyladenosine-modified circIGF2BP3 inhibits CD8+ T-cell responses to facilitate tumor immune evasion by promoting the deubiquitination of PD-L1 in non-small cell lung cancer[J]. Molecular cancer, 2021, 20(1): 1-25.（IF 27.401）
good2. Xuan Q, Jiang F, Dong H, et al. Bioinspired Intrinsic Versatile Hydrogel Fabricated by Amyloidal Toxin Simulant‐Based Nanofibrous Assemblies for Accelerated Diabetic Wound Healing[J]. Advanced Functional Materials, 2021, 31(49): 2106705.（IF 18.808）
good3. Ba F, Liu Y, Liu W Q, et al. SYMBIOSIS: synthetic manipulable biobricks via orthogonal serine integrase systems[J]. Nucleic acids research, 2022, 50(5): 2973-2985.（IF 16.971）
good4. Liu Z, Wu K, Gu S, et al. A methyltransferase‐like 14/miR‐99a‐5p/tribble 2 positive feedback circuit promotes cancer stem cell persistence and radioresistance via histone deacetylase 2‐mediated epigenetic modulation in esophageal squamous cell carcinoma[J]. Clinical and translational medicine, 2021, 11(9): e545.（IF 11.492）
good5. Hu Q, Yao J, Wu X, et al. Emodin attenuates severe acute pancreatitis-associated acute lung injury by suppressing pancreatic exosome-mediated alveolar macrophage activation[J]. Acta Pharmaceutica Sinica B, 2021.（IF 11.413）
good6. He, Q., Wu, Z., Yang, W., Jiang, D., Hu, C., Yang, X., ... & Li, F. (2020). IFI16 promotes human embryonic stem cell trilineage specification through interaction with p53. NPJ Regenerative Medicine, 5(1), 1-14. （IF 10.364）
good7. Ma S, Gu X, Shen L, et al. CircHAS2 promotes the proliferation, migration, and invasion of gastric cancer cells by regulating PPM1E mediated by hsa-miR-944[J]. Cell death & disease, 2021, 12(10): 1-12.（IF 8.469）
good8. ZHENG, Huo-Liang, et al. Beraprost ameliorates postmenopausal osteoporosis by regulating Nedd4-induced Runx2 ubiquitination. Cell death & disease, 2021, 12.5: 1-16. （IF 8.469）
good9. Liu, H., Tian, Z., Guo, Y., Liu, X., Ma, Y., Du, X., ... & Zhang, H. (2020). Microcystin-leucine arginine exposure contributes to apoptosis and follicular atresia in mice ovaries by endoplasmic reticulum stress-upregulated Ddit3. Science of The Total Environment, 144070. （IF 7.963）
good10. Hua T, Wang H, Fan X, et al. BRD4 Inhibition Attenuates Inflammatory Pain by Ameliorating NLRP3 Inflammasome-Induced Pyroptosis[J]. Frontiers in immunology, 2022, 13: 837977-837977.（IF 7.561）
good11. CHEN, Binzhen, et al. MicroLet-7b Regulates Neutrophil Function and Dampens Neutrophilic Inflammation by Suppressing the Canonical TLR4/NF-κB Pathway. Frontiers in immunology, 2021, 12: 856.（IF 7.561）
good12. Li, D., Mao, C., Zhou, E., You, J., Gao, E., Han, Z., ... & Wang, C. (2020). MicroRNA-21 Mediates a Positive Feedback on Angiotensin II-Induced Myofibroblast Transformation. Journal of Inflammation Research, 13, 1007. （IF 6.922）
good13. Hu Q, Wei W, Wu D, et al. Blockade of GCH1/BH4 Axis Activates Ferritinophagy to Mitigate the Resistance of Colorectal Cancer to Erastin-Induced Ferroptosis[J]. Frontiers in cell and developmental biology, 2022, 10: 810327-810327.（IF 6.684）
good14. Tang X, Sun Y, Xu C, et al. Caffeinie Induces Autophagy and Apoptosis in Auditory Hair Cells via the SGK1/HIF-1α Pathway[J]. Frontiers in cell and developmental biology, 2021, 9.（IF 6.684）
good15. ZHOU, Xiang, et al. Prenatal exposure to di-n-butyl phthalate induces erectile dysfunction in male adult rats. Ecotoxicology and Environmental Safety, 2021, 219: 112323.（IF 6.291）
good16. Xu L F, Shi L, Zhang S S, et al. LukS-PV Induces Apoptosis via the SET8-H4K20me1-PIK3CB Axis in Human Acute Myeloid Leukemia Cells[J]. Frontiers in oncology, 2021, 11.（IF 6.244）
good17. CHEN, Zhengqian, et al. HOXA13, negatively regulated by miR-139-5p, decreases the sensitivity of gastric cancer to 5-Fluorouracil possibly by targeting ABCC4. Frontiers in Oncology, 2021, 11.（IF 6.244）
good18. Zhong Y, Wang K, Zhang Y, et al. Ocular Wnt/β-Catenin Pathway Inhibitor XAV939-Loaded Liposomes for Treating Alkali-Burned Corneal Wound and Neovascularization[J]. Frontiers in Bioengineering and Biotechnology, 2021, 9.（IF 5.890）
good19. LIU, Rong, et al. Flotillin-2 promotes cell proliferation via activating the c-Myc/BCAT1 axis by suppressing miR-33b-5p in nasopharyngeal carcinoma. Aging (Albany NY), 2021, 13.6: 8078.（IF 5.682）
good20. DING, Tao, et al. Vitexin exerts protective effects against calcium oxalate crystal-induced kidney pyroptosis in vivo and in vitro. Phytomedicine, 2021, 86: 153562.（IF 5.340）
good2. Xuan Q, Jiang F, Dong H, et al. Bioinspired Intrinsic Versatile Hydrogel Fabricated by Amyloidal Toxin Simulant‐Based Nanofibrous Assemblies for Accelerated Diabetic Wound Healing[J]. Advanced Functional Materials, 2021, 31(49): 2106705.（IF 18.808）
good3. Ba F, Liu Y, Liu W Q, et al. SYMBIOSIS: synthetic manipulable biobricks via orthogonal serine integrase systems[J]. Nucleic acids research, 2022, 50(5): 2973-2985.（IF 16.971）
good4. Liu Z, Wu K, Gu S, et al. A methyltransferase‐like 14/miR‐99a‐5p/tribble 2 positive feedback circuit promotes cancer stem cell persistence and radioresistance via histone deacetylase 2‐mediated epigenetic modulation in esophageal squamous cell carcinoma[J]. Clinical and translational medicine, 2021, 11(9): e545.（IF 11.492）
good5. Hu Q, Yao J, Wu X, et al. Emodin attenuates severe acute pancreatitis-associated acute lung injury by suppressing pancreatic exosome-mediated alveolar macrophage activation[J]. Acta Pharmaceutica Sinica B, 2021.（IF 11.413）
good6. He, Q., Wu, Z., Yang, W., Jiang, D., Hu, C., Yang, X., ... & Li, F. (2020). IFI16 promotes human embryonic stem cell trilineage specification through interaction with p53. NPJ Regenerative Medicine, 5(1), 1-14. （IF 10.364）
good7. Ma S, Gu X, Shen L, et al. CircHAS2 promotes the proliferation, migration, and invasion of gastric cancer cells by regulating PPM1E mediated by hsa-miR-944[J]. Cell death & disease, 2021, 12(10): 1-12.（IF 8.469）
good8. ZHENG, Huo-Liang, et al. Beraprost ameliorates postmenopausal osteoporosis by regulating Nedd4-induced Runx2 ubiquitination. Cell death & disease, 2021, 12.5: 1-16. （IF 8.469）
good9. Liu, H., Tian, Z., Guo, Y., Liu, X., Ma, Y., Du, X., ... & Zhang, H. (2020). Microcystin-leucine arginine exposure contributes to apoptosis and follicular atresia in mice ovaries by endoplasmic reticulum stress-upregulated Ddit3. Science of The Total Environment, 144070. （IF 7.963）
good10. Hua T, Wang H, Fan X, et al. BRD4 Inhibition Attenuates Inflammatory Pain by Ameliorating NLRP3 Inflammasome-Induced Pyroptosis[J]. Frontiers in immunology, 2022, 13: 837977-837977.（IF 7.561）
good11. CHEN, Binzhen, et al. MicroLet-7b Regulates Neutrophil Function and Dampens Neutrophilic Inflammation by Suppressing the Canonical TLR4/NF-κB Pathway. Frontiers in immunology, 2021, 12: 856.（IF 7.561）
good12. Li, D., Mao, C., Zhou, E., You, J., Gao, E., Han, Z., ... & Wang, C. (2020). MicroRNA-21 Mediates a Positive Feedback on Angiotensin II-Induced Myofibroblast Transformation. Journal of Inflammation Research, 13, 1007. （IF 6.922）
good13. Hu Q, Wei W, Wu D, et al. Blockade of GCH1/BH4 Axis Activates Ferritinophagy to Mitigate the Resistance of Colorectal Cancer to Erastin-Induced Ferroptosis[J]. Frontiers in cell and developmental biology, 2022, 10: 810327-810327.（IF 6.684）
good14. Tang X, Sun Y, Xu C, et al. Caffeinie Induces Autophagy and Apoptosis in Auditory Hair Cells via the SGK1/HIF-1α Pathway[J]. Frontiers in cell and developmental biology, 2021, 9.（IF 6.684）
good15. ZHOU, Xiang, et al. Prenatal exposure to di-n-butyl phthalate induces erectile dysfunction in male adult rats. Ecotoxicology and Environmental Safety, 2021, 219: 112323.（IF 6.291）
good16. Xu L F, Shi L, Zhang S S, et al. LukS-PV Induces Apoptosis via the SET8-H4K20me1-PIK3CB Axis in Human Acute Myeloid Leukemia Cells[J]. Frontiers in oncology, 2021, 11.（IF 6.244）
good17. CHEN, Zhengqian, et al. HOXA13, negatively regulated by miR-139-5p, decreases the sensitivity of gastric cancer to 5-Fluorouracil possibly by targeting ABCC4. Frontiers in Oncology, 2021, 11.（IF 6.244）
good18. Zhong Y, Wang K, Zhang Y, et al. Ocular Wnt/β-Catenin Pathway Inhibitor XAV939-Loaded Liposomes for Treating Alkali-Burned Corneal Wound and Neovascularization[J]. Frontiers in Bioengineering and Biotechnology, 2021, 9.（IF 5.890）
good19. LIU, Rong, et al. Flotillin-2 promotes cell proliferation via activating the c-Myc/BCAT1 axis by suppressing miR-33b-5p in nasopharyngeal carcinoma. Aging (Albany NY), 2021, 13.6: 8078.（IF 5.682）
good20. DING, Tao, et al. Vitexin exerts protective effects against calcium oxalate crystal-induced kidney pyroptosis in vivo and in vitro. Phytomedicine, 2021, 86: 153562.（IF 5.340）
上海雅酶生物医药科技有限公司
品牌商实名认证
钻石会员
入驻年限：7年
