产品详情
文献和实验
研选推荐
保质期 ：一年
保存条件 ：保存于4℃，其中改良型促凝剂保存于-20℃。
规格 ：125gel（0.75mm）
PAGE凝胶快速制备试剂盒Color PAGE Gel Rapid Preparation Kit
本产品常温运输；保存于4℃，其中 改良型促凝剂 保存于-20℃，保质期12个月。
货号规格
| 货号 | 可制胶数量 |
| PG110(制备6%的PAGE胶) PG111(制备7.5%的PAGE胶) PG112(制备10%的PAGE胶) PG113(制备12.5%的PAGE胶) PG114(制备15%的PAGE胶) |
125块(0.75 mm胶) or ＞90块(1.00 mm胶) or ＞60块(1.50 mm胶) |
产品内容
| 组分名称 | 体积及数量 |
| 上层胶溶液(2×) | 80 mL |
| 彩色上层胶缓冲液(2×) | 80 mL |
| 下层胶溶液(2×) | 250 mL |
| 下层胶缓冲液(2×) | 250 mL |
| 改良型促凝剂 | 8 mL |
产品特点
g
d快速制备凝胶——短时间即可灌制多块凝胶，无需计算所需溶液量，无需稀释；g
d彩色上层胶——可制备红蓝绿三种颜色的上层胶，为点样和区分不同凝胶提供便利；g
d避免异味——无需使用TEMED，避免恶臭气味；g
d条带清晰——尤其小分子蛋白质条带比在传统凝胶中更清晰。产品简介
good本产品适用于Tris-甘氨酸电泳体系，本产品采用上层胶和下层胶的预混配方，只需加入 改良型促凝剂 即可凝胶，简便快捷。所配的上层胶带有颜色(红色、蓝色或绿色)，点样孔清晰易辨，方便点样。三种颜色设计，可用于区分含不同样品的凝胶。本试剂盒灌制的凝胶也可用于非变性PAGE凝胶电泳。
good本产品配套提供 改良型促凝剂 ，其具有更好的稳定性和催化效能，配胶过程中无需额外添加TEMED。为方便操作，已开盖使用中的 改良型促凝剂 可置于4℃保存至少三个月。
制胶流程(以一块0.75/1.0/1.5 mm的mini胶为例)
good1. 取等体积 下层胶溶液 和 下层胶缓冲液 ，各2.0/2.7/4.0 mL，混匀；
good2. 向步骤1的混合溶液中加入40/60/80 μL的 改良型促凝剂 ，混匀；
good4. 注意：加入改良型促凝剂后，需轻柔混匀，防止过多氧气混入胶溶液，抑制凝胶聚合。
good3. 将步骤2的混合溶液注入制胶玻璃板中，使液面和短玻璃板上沿之间的距离比梳齿长0.5 cm即可(注意：此溶液为过量，请勿全部注入，可留少许于配胶杯中，以判断胶凝固状况)，加入适量水或醇(如异丙醇、正丁醇等)覆盖于下层胶之上；
good4. 待下层胶凝固后(约15 min)，倒去上层水或醇；
good4. 注意：当水(醇)和胶之间有一条折射线时，说明胶已凝固。
good5. 取等体积 上层胶溶液 和 彩色上层胶缓冲液 ，各0.5/0.75/1.0 mL，混匀；
good4. 注意：由于染料的特殊理化性质，使用前请摇匀。
good6. 向步骤5的混合溶液中加入10/15/20 μL的 改良型促凝剂 ，混匀；
good4. 注意：加入改良型促凝剂后，需轻柔混匀，防止过多氧气混入胶溶液，抑制凝胶聚合。
good7. 将步骤6的混合溶液注入制胶玻璃板中，插入梳齿；
good8. 待上层胶凝固后(约15 min)，拔去梳齿即可用于电泳。
good6. 注意：请尽量使用新鲜配制的电泳缓冲液。
| 下层胶配方 | 上层胶配方 | |||||||
| 凝 胶 厚 度 |
下层胶溶 液 | 下层胶缓冲液 | 改良型促凝剂 | 凝 胶 厚 度 |
上层胶溶 液 | 上层胶缓冲液 | 改良型促凝剂 | |
| 0.75 mm | 2.0 mL | 2.0 mL | 40 μL | 0.75 mm | 0.5 mL | 0.5 mL | 10 μL | |
| 1.00 mm | 2.7 mL | 2.7 mL | 60 μL | 1.00 mm | 0.75 mL | 0.75 mL | 15 μL | |
| 1.50 mm | 4.0 mL | 4.0 mL | 80 μL | 1.50 mm | 1.0 mL | 1.0 mL | 20 μL | |
注意事项
good1. 本产品制备出的凝胶其上层胶对样品没有浓缩效应，与预制胶类似，但与传统PAGE胶相比，对蛋白条带分离效果更好，小分子量蛋白(比如10 kDa)也可以清晰地分离开，且蛋白条带更窄更锐利；
good2. 改良型促凝剂的使用量仅作参考，实际用量可根据个人实验习惯和经验调整。加入较多量的促凝剂可加速凝胶，反之亦然；
good3. 凝胶速度与温度有显著的正相关性。同等条件下，温度越高，凝胶速度越快，室温过高时建议适当减小改良型促凝剂的用量；相反，如果室温较低，可适当延长凝胶时间；
good4. 本产品已加入适量TEMED的替代品，如需进一步加速凝胶，临配胶前可按需补充适量TEMED；
good5. 在配胶之前，使胶溶液及缓冲液平衡到室温(如室温放置几分钟)，可有效避免凝胶中气泡的形成；
good6. 推荐电泳条件为：150 V，约50 min(或200 V，约35 min)；
good7. 为了您的安全和健康，请穿实验服并戴一次性手套操作；
good8. 本产品仅限科研使用。
凝胶浓度选择参考
相关论文
good1. Zhao, Q., Liu, J., Deng, H., Ma, R., Liao, J. Y., Liang, H., ... & Xu, X. (2020). Targeting mitochondria-located circRNA SCAR alleviates NASH via reducing mROS output. Cell, 183(1), 76-93.(IF 41.582)
good2. ZHENG, Xiaojiao, et al. Hyocholic acid species improve glucose homeostasis through a distinct TGR5 and FXR signaling mechanism. Cell metabolism, 2021, 33.4: 791-803. e7.（IF 27.287）
good3. Yue, Y., Xu, W., Kan, Y., Zhao, H. Y., Zhou, Y., Song, X., ... & Lamriben, L. (2020). Extensive germline genome engineering in pigs. Nature Biomedical Engineering, 1-10.(IF 25.671)
good4. YUE, Yanan, et al. Extensive germline genome engineering in pigs. Nature Biomedical Engineering, 2021, 5.2: 134-143.(IF 25.671)
good5. DU, Xufei, et al. Eosinophil-derived chemokine (hCCL15/23, mCCL6) interacts with CCR1 to promote eosinophilic airway inflammation. Signal transduction and targeted therapy, 2021, 6.1: 1-11.(IF 18.187)
good6. Li S, Chen J, Liu M, et al. Protective effect of HINT2 on mitochondrial function via repressing MCU complex activation attenuates cardiac microvascular ischemia–reperfusion injury[J]. Basic research in cardiology, 2021, 116(1): 1-18.（IF 17.165）
good7.Ba F, Liu Y, Liu W Q, et al. SYMBIOSIS: synthetic manipulable biobricks via orthogonal serine integrase systems[J]. Nucleic acids research, 2022, 50(5): 2973-2985.（IF 16.971）
good8. DU, Xufei, et al. Eosinophil-derived chemokine (hCCL15/23, mCCL6) interacts with CCR1 to promote eosinophilic airway inflammation. Signal transduction and targeted therapy, 2021, 6.1: 1-11.(IF 16.016)
good9.Liu C, Yang Y, Chen C, et al. Environmental eustress modulates β-ARs/CCL2 axis to induce anti-tumor immunity and sensitize immunotherapy against liver cancer in mice[J]. Nature Communications, 2021, 12(1): 1-15.（IF 14.919）
good10. Wei, Y., Tian, C., Zhao, Y., Liu, X., Liu, F., Li, S., ... & Zhou, T. (2020). MRG15 orchestrates rhythmic epigenomic remodelling and controls hepatic lipid metabolism. Nature Metabolism, 2(5), 447-460. (IF 13.511)
good11.Wu B, Ye Y, Xie S, et al. Megakaryocytes mediate hyperglycemia-induced tumor metastasis[J]. Cancer Research, 2021, 81(21): 5506-5522.（IF 12.701）
good12. Ma, W., Zhu, D., Li, J., Chen, X., Xie, W., Jiang, X., ... & Wang, F. (2020). Coating biomimetic nanoparticles with chimeric antigen receptor T cell-membrane provides high specificity for hepatocellular carcinoma photothermal therapy treatment. Theranostics, 10(3), 1281.(IF 11.556)
good13. TAN, Xingliang, et al. Molecular stratification by BCL2A1 and AIM2 provides additional prognostic value in penile squamous cell carcinoma. Theranostics, 2021, 11.3: 1364.(IF 11.556)
good14. YANG, Yang, et al. Rebalancing TGF‐β/Smad7 signaling via Compound kushen injection in hepatic stellate cells protects against liver fibrosis and hepatocarcinogenesis. Clinical and Translational Medicine, 2021, 11.7: e410.(IF 11.492)
good15. Yang, L., Li, Y., Gong, R., Gao, M., Feng, C., Liu, T., ... & Yan, G. (2019). The long non-coding RNA-ORLNC1 regulates bone mass by directing mesenchymal stem cell fate. Molecular Therapy, 27(2), 394-410.(IF 11.454)
good16. Yang, M., Liu, X., Luo, Q., Xu, L., & Chen, F. (2020). An ef ficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy. Journal of nanobiotechnology, 18(1), 1-12.(IF 10.435)
good17. LIU, Yingjun, et al. A heparin–rosuvastatin-loaded P (LLA-CL) nanofiber-covered stent inhibits inflammatory smooth-muscle cell viability to reduce in-stent stenosis and thrombosis. Journal of nanobiotechnology, 2021, 19.1: 1-17.(IF 10.435)
good18. Wang, Z., Chi, D., Wu, X., Wang, Y., Lin, X., Xu, Z., ... & Wang, Y. (2019). Tyrosine modified irinotecan-loaded liposomes capable of simultaneously targeting LAT1 and ATB0,+ for eff icient tumor therapy. Journal of Controlled Release, 316, 22-33.(IF 9.776)
good19. SUN, Jie, et al. Synergistically Bifunctional Paramagnetic Separation Enables Ef ficient Isolation of Urine Extracellular Vesicles and Downstream Phosphoproteomic Analysis. ACS Applied Materials & Interfaces, 2021, 13.3: 3622-3630.（IF 9.229）
good20. LIN, Junhao, et al. EFNA4 promotes cell proliferation and tumor metastasis in hepatocellular carcinoma through a PIK3R2/GSK3β/β-catenin positive feedback loop. Molecular Therapy-Nucleic Acids, 2021.（IF 8.886）
good21. Gu, C., Yang, H., Chang, K., Zhang, B., Xie, F., Ye, J., ... & Wang, J. (2020). Melatonin alleviates progression of uterine endometrial cancer by suppressing estrogen/ubiquitin C/SDHB-mediated succinate accumulation. Cancer Letters, 476, 34-47.（IF 8.679）
good22.Cui H, Jiang Z, Zeng S, et al. A new candidate oncogenic lncRNA derived from pseudogene WFDC21P promotes tumor progression in gastric cancer[J]. Cell death & disease, 2021, 12(10): 1-12.（IF 8.469）
good23.Wang X, Jin P, Zhang Y, et al. CircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and interacting with microRNAs[J]. Cell death & disease, 2021, 12(4): 1-13.（IF 8.469）
good24. Zhang, B., Zhou, W. J., Gu, C. J., Wu, K., Yang, H. L., Mei, J., ... & Li, D. J. (2018). The ginsenoside PPD exerts anti-endometriosis effects by suppressing estrogen receptor-mediated inhibition of endometrial stromal cell autophagy and NK cell cytotoxicity. Cell death & disease, 9(5), 1-13.（IF 8.469）
good25. Zhu, B., Xue, F., Zhang, C., & Li, G. (2019). LMCD1 promotes osteogenic differentiation of human bone marrow stem cells by regulating BMP signaling. Cell Death & Disease, 10(9), 1-11.（IF 8.469）
good2. ZHENG, Xiaojiao, et al. Hyocholic acid species improve glucose homeostasis through a distinct TGR5 and FXR signaling mechanism. Cell metabolism, 2021, 33.4: 791-803. e7.（IF 27.287）
good3. Yue, Y., Xu, W., Kan, Y., Zhao, H. Y., Zhou, Y., Song, X., ... & Lamriben, L. (2020). Extensive germline genome engineering in pigs. Nature Biomedical Engineering, 1-10.(IF 25.671)
good4. YUE, Yanan, et al. Extensive germline genome engineering in pigs. Nature Biomedical Engineering, 2021, 5.2: 134-143.(IF 25.671)
good5. DU, Xufei, et al. Eosinophil-derived chemokine (hCCL15/23, mCCL6) interacts with CCR1 to promote eosinophilic airway inflammation. Signal transduction and targeted therapy, 2021, 6.1: 1-11.(IF 18.187)
good6. Li S, Chen J, Liu M, et al. Protective effect of HINT2 on mitochondrial function via repressing MCU complex activation attenuates cardiac microvascular ischemia–reperfusion injury[J]. Basic research in cardiology, 2021, 116(1): 1-18.（IF 17.165）
good7.Ba F, Liu Y, Liu W Q, et al. SYMBIOSIS: synthetic manipulable biobricks via orthogonal serine integrase systems[J]. Nucleic acids research, 2022, 50(5): 2973-2985.（IF 16.971）
good8. DU, Xufei, et al. Eosinophil-derived chemokine (hCCL15/23, mCCL6) interacts with CCR1 to promote eosinophilic airway inflammation. Signal transduction and targeted therapy, 2021, 6.1: 1-11.(IF 16.016)
good9.Liu C, Yang Y, Chen C, et al. Environmental eustress modulates β-ARs/CCL2 axis to induce anti-tumor immunity and sensitize immunotherapy against liver cancer in mice[J]. Nature Communications, 2021, 12(1): 1-15.（IF 14.919）
good10. Wei, Y., Tian, C., Zhao, Y., Liu, X., Liu, F., Li, S., ... & Zhou, T. (2020). MRG15 orchestrates rhythmic epigenomic remodelling and controls hepatic lipid metabolism. Nature Metabolism, 2(5), 447-460. (IF 13.511)
good11.Wu B, Ye Y, Xie S, et al. Megakaryocytes mediate hyperglycemia-induced tumor metastasis[J]. Cancer Research, 2021, 81(21): 5506-5522.（IF 12.701）
good12. Ma, W., Zhu, D., Li, J., Chen, X., Xie, W., Jiang, X., ... & Wang, F. (2020). Coating biomimetic nanoparticles with chimeric antigen receptor T cell-membrane provides high specificity for hepatocellular carcinoma photothermal therapy treatment. Theranostics, 10(3), 1281.(IF 11.556)
good13. TAN, Xingliang, et al. Molecular stratification by BCL2A1 and AIM2 provides additional prognostic value in penile squamous cell carcinoma. Theranostics, 2021, 11.3: 1364.(IF 11.556)
good14. YANG, Yang, et al. Rebalancing TGF‐β/Smad7 signaling via Compound kushen injection in hepatic stellate cells protects against liver fibrosis and hepatocarcinogenesis. Clinical and Translational Medicine, 2021, 11.7: e410.(IF 11.492)
good15. Yang, L., Li, Y., Gong, R., Gao, M., Feng, C., Liu, T., ... & Yan, G. (2019). The long non-coding RNA-ORLNC1 regulates bone mass by directing mesenchymal stem cell fate. Molecular Therapy, 27(2), 394-410.(IF 11.454)
good16. Yang, M., Liu, X., Luo, Q., Xu, L., & Chen, F. (2020). An ef ficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy. Journal of nanobiotechnology, 18(1), 1-12.(IF 10.435)
good17. LIU, Yingjun, et al. A heparin–rosuvastatin-loaded P (LLA-CL) nanofiber-covered stent inhibits inflammatory smooth-muscle cell viability to reduce in-stent stenosis and thrombosis. Journal of nanobiotechnology, 2021, 19.1: 1-17.(IF 10.435)
good18. Wang, Z., Chi, D., Wu, X., Wang, Y., Lin, X., Xu, Z., ... & Wang, Y. (2019). Tyrosine modified irinotecan-loaded liposomes capable of simultaneously targeting LAT1 and ATB0,+ for eff icient tumor therapy. Journal of Controlled Release, 316, 22-33.(IF 9.776)
good19. SUN, Jie, et al. Synergistically Bifunctional Paramagnetic Separation Enables Ef ficient Isolation of Urine Extracellular Vesicles and Downstream Phosphoproteomic Analysis. ACS Applied Materials & Interfaces, 2021, 13.3: 3622-3630.（IF 9.229）
good20. LIN, Junhao, et al. EFNA4 promotes cell proliferation and tumor metastasis in hepatocellular carcinoma through a PIK3R2/GSK3β/β-catenin positive feedback loop. Molecular Therapy-Nucleic Acids, 2021.（IF 8.886）
good21. Gu, C., Yang, H., Chang, K., Zhang, B., Xie, F., Ye, J., ... & Wang, J. (2020). Melatonin alleviates progression of uterine endometrial cancer by suppressing estrogen/ubiquitin C/SDHB-mediated succinate accumulation. Cancer Letters, 476, 34-47.（IF 8.679）
good22.Cui H, Jiang Z, Zeng S, et al. A new candidate oncogenic lncRNA derived from pseudogene WFDC21P promotes tumor progression in gastric cancer[J]. Cell death & disease, 2021, 12(10): 1-12.（IF 8.469）
good23.Wang X, Jin P, Zhang Y, et al. CircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and interacting with microRNAs[J]. Cell death & disease, 2021, 12(4): 1-13.（IF 8.469）
good24. Zhang, B., Zhou, W. J., Gu, C. J., Wu, K., Yang, H. L., Mei, J., ... & Li, D. J. (2018). The ginsenoside PPD exerts anti-endometriosis effects by suppressing estrogen receptor-mediated inhibition of endometrial stromal cell autophagy and NK cell cytotoxicity. Cell death & disease, 9(5), 1-13.（IF 8.469）
good25. Zhu, B., Xue, F., Zhang, C., & Li, G. (2019). LMCD1 promotes osteogenic differentiation of human bone marrow stem cells by regulating BMP signaling. Cell Death & Disease, 10(9), 1-11.（IF 8.469）
上海雅酶生物医药科技有限公司
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钻石会员
入驻年限：7年
