Anti-VWA5A antibody

供应商 ：江西江蓝纯生物试剂有限公司

库存 ：大量

克隆性 ：多克隆

保质期 ：1年

抗体英文名 ：Anti-VWA5A antibody

宿主 ：Rabbit

浓度 ：0.5 mg/ml

保存条件 ：-20°C

规格 ：25 μl/100 μl/200 μl

Anti-VWA5A antibody

Cat. No.     JLC221980        
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-VWA5A rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Fusion protein of human VWA5A
Reactivity                 Human
Content                     0.5 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 VWA5A
Full name                 von Willebrand factor A domain containing 5A
Synonyms    BCSC1; BCSC-1; LOH11CR2A
Swissprot    O00534

Target Background
VWA5A (von Willebrand factor A domain containing 5A), also known as BCSC-1 (breast cancer suppressor candidate 1) or LOH11CR2A (loss of heterozygosity 11 chromosomal region 2 gene A protein), is a 786 amino acid protein containing one VIT domain and one VWFA domain. VWA5A is expressed at low levels in various tissues, with no expression found in 80% of tumor cell lines. Likely acting as a tumor suppressor gene, deletion of VWA5A leads to loss of heterozygosity (LOH) in breast and ovarian tumors, and may have an important role as a potential gene therapy target. Abnormal expression of VWA5A may lead to an increase in adhesion of CNE-2L2 cells associated with an increase in expression of E-cadherin, alpha-catenin, and p53, resulting in a decrease of malignant activity in cells with ectopic expression of VWA5A.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human lung cancer
Recommended dilution: 100-300    Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 100-300
            
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ml221980(VWA5A Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml221980(VWA5A Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:86 kDa
Positive control:Human Adrenal pheochromocytoma tissue,Jurkat and A549 cells 
 
Recommended dilution: 500-2000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane 1-3: Human Adrenal pheochromocytoma tissue, Jurkat cells, A549 cells
Primary antibody: ml221980(VWA5A Antibody) at dilution 1/275
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 10 seconds
    
ELISA
Recommended dilution: 2000-10000