FITC标记的磷酸化p21激活激酶1/2/3抗体

供应商 ：上海联迈生物工程有限公司

库存 ：大量

靶点 ：详见说明书

级别 ：1

目录编号 ：LM-1652R-FITC

克隆性 ：多克隆

抗原来源 ：Rabbit

保质期 ：1年

抗体英文名 ：Anti-phospho-PAK1/2/3(Thr423)/FITC

抗体名 ：Anti-phospho-PAK1/2/3(Thr423)/FITC

标记物 ：FITC标记

宿主 ：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,

适应物种 ：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,

免疫原 ：详见说明书

亚型 ：IGg

形态 ：粉末、液体、冻干粉

应用范围 ：IF=1:50-200

浓度 ：1mg/ml

保存条件 ：-20 °C

规格 ：100ul

FITC标记的磷酸化p21激活激酶1/2/3抗体

英文名称	Anti-phospho-PAK1/2/3(Thr423)/FITC
中文名称	FITC标记的磷酸化p21激活激酶1/2/3抗体
别    名	PAK1 + PAK2 + PAK3 (phospho T423); Alpha PAK; Beta PAK; Gamma PAK; Oligophrenin 3; OPHN3; p21 activated kinase 1; p21 Activated Kinase 1 + 2 + 3; p21 activated kinase 2 ; p21 activated kinase 3; P65 PAK; PAK1; PAK2; PAK3; PAK3beta; PAK65; PAKalpha; PAKgamma; S6/H4 kinase; Serine/threonine protein kinase PAK 1; Serine/threonine protein kinase PAK 2; Serine/threonine protein kinase PAK 3; Serine/threonine protein kinase PAK1; Serine/threonine protein kinase PAK2; Serine/threonine protein kinase PAK3; PAK1_HUMAN; PAK2_HUMAN; PAK3_HUMAN.
规格价格	100ul/2980元 购买        大包装/询价
说 明 书	100ul
产品类型	磷酸化抗体
研究领域	肿瘤  细胞生物  神经生物学  信号转导  细胞凋亡  转录调节因子  激酶和磷酸酶
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
产品应用	IF=1:50-200   not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	60kDa
细胞定位	细胞膜
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated Synthesised phosphopeptide derived from human PAK1 around the phosphorylation site of (Thr423)
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍	background: The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK. Function: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Subunit: Homodimer in its autoinhibited state. Active as monomer. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. Interacts with ARHGEF7. Also interacts with CRIPAK. Interacts with NISCH. Interacts with DVL1; mediates the formation of a DVL1, MUSK and PAK1 ternary complex involved in AChR clustering. Probably found in a ternary complex composed of DSCAM, PAK1 and RAC1. Interacts with DSCAM (via cytoplasmic domain); the interaction is direct and enhanced in presence of RAC1. Interacts with SCRIB. Interacts with PDPK1. Interacts (via kinase domain) with RAF1. Interaction with NCK1 and NCK2. Subcellular Location: Cytoplasm. Cell junction, focal adhesion. Note=Recruited to focal adhesions upon activation. Post-translational modifications: Autophosphorylated when activated by CDC42/p21 and RAC1. Phosphorylation at Thr-423 by PDPK1 results in its activation. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain.  Contains 1 protein kinase domain. Database links: Entrez Gene: 5058 Human Entrez Gene: 18479 Mouse Entrez Gene: 29431 Rat Omim: 602590 Human SwissProt: Q13153 Human SwissProt: O88643 Mouse SwissProt: P35465 Rat Unigene: 435714 Human Unigene: 260227 Mouse Unigene: 9149 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.  PAK1-3蛋白具有广泛的生物学功能,也是一个保守的丝氨酸/苏氨酸蛋白激酶,参与许多重要的细胞活动。包括细胞骨架的动力学调节,细胞移动,生存和凋亡,细胞周期,基因转录调节,细胞生长信号转导和转化等。